PE anti-mouse CD180 (RP105) Antibody

Pricing & Availability
Clone
RP/14 (See other available formats)
Regulatory Status
RUO
Other Names
CD180, Radio-protective 105-kD protein, RP105
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RPslash14_PE_090507
C57BL/6 mouse splenocytes stained with RP/14 PE
  • RPslash14_PE_090507
    C57BL/6 mouse splenocytes stained with RP/14 PE
See PE spectral data
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117706 200 µg 384 CHF
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Description

CD180 is a 105 kD type I membrane protein, also known as RP105 or Ly64. It is a member of the toll-like receptor family consisting of an extracellular leucine-rich repeat (LRR) and a short cytoplasmic tail. It is expressed on most B cells, monocytes/macrophages, and dendritic cells. CD180 associates with MD-1 to form the RP105/MD-1 surface receptor. CD180/MD-1, by working in concert with TLR4, controls B cell recognition and signalling of bacterial LPS. The ligation of RP105 by RP/14 leads to a strong B cell proliferative response, as well as protection from apoptosis induced by radiation or dexamethasone. Interestingly, in some systems, ligation of RP105 leads to Fas-independent apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, B cell activation1,4,5, protection of B cells from apoptosis induced by radiation or dexamethasone and induction of Fas-independent apoptosis1,5. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 117709 & 117710).

Application References

(PubMed link indicates BioLegend citation)
  1. Miyake K, et al. 1994. J. Exp. Med. 180:1217. (FC IP Activ Apop)
  2. Miyake K, et al. 1995. J. Immunol. 154:3333. (IP)
  3. Miyake K, et al. 1998. J. Immunol. 161:1348. (IP)
  4. Chan VWF, et al. 1998. J. Exp. Med. 188:93. (Activ)
  5. Yamashita Y, et al. 1996. J. Exp. Med. 184:113. (Activ Apop)
  6. Fujita K, et al. 2012. Immunol Lett. 144:1. PubMed.
Product Citations
  1. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  2. Fujita K, et al. 2012. Immunol Lett. 144:1. PubMed
RRID
AB_2072827 (BioLegend Cat. No. 117706)

Antigen Details

Structure
Member of the toll-like receptor family, 105 kD
Distribution

B cells, monocytes/macrophages, and dendritic cells

Function
Signaling protein involved in innate immunity
Ligand/Receptor
LPS
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Miyake K, et al. 1994. J. Exp. Med. 180 :1217.
2. Miyake K, et al. 1995. J. Immunol.. 154:3333.
3. Miyake K, et al. 1998. J. Immunol.. 161:1348.
4. Chan VWF, et al. 1998. J. Exp. Med. 188 :93.
5. Yamashita Y, et al. 1996. J. Exp. Med. 184:113.

Gene ID
17079 View all products for this Gene ID
UniProt
View information about CD180 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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