PE anti-human CD20 Recombinant Antibody

Pricing & Availability
Clone
QA18A14 (See other available formats)
Regulatory Status
RUO
Other Names
B1, Bp35
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A14_PE_CD20_Antibody_081623
Human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human CD20 recombinant (clone QA18A14) PE (left), or mouse IgG1, κ PE isotype control (right).
  • QA18A14_PE_CD20_Antibody_081623
    Human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human CD20 recombinant (clone QA18A14) PE (left), or mouse IgG1, κ PE isotype control (right).
See PE spectral data
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389103 25 tests 85 CHF
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389104 100 tests 170 CHF
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Description

CD20, also known as B1 and Bp35, is a 33-37 kD four transmembrane spanning protein. CD20 is expressed on pre-B cells, resting B cells, and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Human CD20 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3068258 (BioLegend Cat. No. 389103)
AB_3068258 (BioLegend Cat. No. 389104)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Interaction
CD20 associates with CD53, CD81, CD82, MHCII, CD40, CBP, BCR, and Src family kinases
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Molecular Family
CD Molecules
Antigen References
  1. Hultin LE, et al. 1993. Cytometry. 14:196-204.
  2. Tedder T and Engel P. 1994. Immunol Today. 15:450-4.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08.16.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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