PE anti-human CD165 Antibody

Pricing & Availability
Clone
SN2 (N6-D11) (See other available formats)
Regulatory Status
RUO
Workshop
VI N-L099
Other Names
SN2, gp37, AD2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SN2_PE_042408
Human T-lymphoblastic leukemia cell line Jurkat stained with SN2 PE
  • SN2_PE_042408
    Human T-lymphoblastic leukemia cell line Jurkat stained with SN2 PE
See PE spectral data
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329010 100 tests 242 CHF
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Description

CD165 is a 37-42 kD transmembrane glycoprotein, also known as AD2, gp37, and SN2. It is expressed on immature thymocytes, most platelets, thymic epithelial cells, fibroblasts, and acute T-lymphoblastic leukemia (T-ALL) cells. CD165 has also been found to be expressed on monocytes and circulating T cells. In addition, CD165 has been detected on islet cells of the pancreas, Bowman's capsule of the kidney and on central nervous system neurons. SN2 antigen has been reported to be identical, or very similar, to the T-cell acute lymphoblastic leukemia antigen, TALLA-1. The biological function of CD165 is still not clear, though it has been proposed that CD165 plays a role in cell adhesion between thymocytes and thymic epithelial cells during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
purified human CD165 antigen from Molt-4 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen tissue sections.1, Western Blotting1, and immunofluorescence microscopy1

Application References

(PubMed link indicates BioLegend citation)
  1. Seon BK, et al. 1984. J. Immunol. 132:2089.
RRID
AB_2072596 (BioLegend Cat. No. 329010)

Antigen Details

Distribution

immature thymocytes, acute T-lymphoblastic leukemia (T-ALL) cells, epithelial cells, fibroblasts, platelets, monocytes and circulating T cells

Cell Type
Epithelial cells, Fibroblasts, Leukemia, Monocytes, Platelets, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Kishimoto T, et al. 1997. ed. Leucocyte Typing VI:White Cell Differentiation Antigens. London:Garland Publishing.
2. Seon BK. 1995. Int. J. Cancer. 63:474.
3. Takagi S, et al. 1995. Int. J. Cancer. 61:706.

Gene ID
NA
UniProt
View information about CD165 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09.01.2022

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD165

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