LEGENDplex™ Human Diabesity Panel - S/P (4-plex) with Filter Plate

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Other Names
Diabesity, Multiplex, flow cytometry, PAI-1, Insulin, Leptin, Cortisol
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740913 100 tests 650 CHF
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Description

The Human Diabesity Panel is a multiplex bead-based assay panel, which uses fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows for the simultaneous quantification of 11 human proteins involved in diabesity, including PAI-1, GLP-1 (Total), Insulin, C-Peptide, TNFα, Glucagon, Leptin, Cortisol, IL-1β, IL-6 and GLP-1 (Active). This assay panel provides broader dynamic ranges than traditional ELISA methods and has been validated for use with tissue culture supernatant, serum, and EDTA plasma samples.

 

The LEGENDplex™ Human Diabesity Panel is configured as shown below depending on sample types and required dilutions:

 

Catalog No.

Plex Size

Targets

Recommended Sample Type

Recommended Dilution Factor

740909

740910

11-plex

PAI-1, GLP-1 (Total), Insulin, C-Peptide, TNFα, Glucagon, Leptin, Cortisol*, IL-1β, IL-6 and GLP-1 (Active)

Tissue Culture

Varies

740911

740912

7-plex

GLP-1 (Total), C-Peptide, TNFα, Glucagon, IL-1β, IL-6 and GLP-1 (Active)

Serum, Plasma

1:1

740913

740914

4-plex

PAI-1, Insulin, Leptin, Cortisol*

Serum, Plasma

1:8

 

 

* This panel introduces a competitive assay for Cortisol (on B5 bead). A concentrated cortisol tracer is provided in this kit which will need to be diluted 100-fold before the assay. This cortisol tracer will be added to all wells before adding the beads. The competition between the cortisol tracer and the standard cortisol will characterize the standard curve. Due to the nature of the assay, the standard curve will have the highest signal at the lowest standard cortisol concentration and will decrease as the standard cortisol concentration increases.

Note: The cortisol tracer is for the cortisol assay ONLY and does not affect any other target.

The LEGENDplex™ Human Diabesity Panel is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.


This assay is for research use only.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads 1: FITC Beads (1 ml)
  • Setup Beads 2: PE Beads (1 ml)
  • Setup Beads 3: Raw Beads (2 ml)
  • Capture Beads (Varies)
  • Detection Antibodies (3.5 ml)
  • Standard Cocktail, Lyophilized (1 vial)
  • Human Cortisol Tracer, 100X (50 μl)
  • SA-PE (3.5 ml)
  • Assay Buffer (25 ml)
  • Matrix B, Lyophilized (1 vial)
  • Wash Buffer, 20X (25 ml)
  • Plate Sealers (4 sheets)
  • Data Analysis Software Dongle
  • Filter Plate
  • Manual

Product Details

Reactivity
Human
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes
  • Micro FACS tubes (if the flow cytometer does not contain an autosampler)
  • Vortex Mixer
  • Sonicator
  • Aluminum Foil
  • Absorbent Pads or Paper Towels
  • Plate Shaker
  • Tabletop Centrifuges
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)

Related FAQs

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

If I don't have a vacuum, how do I remove the liquid from my plate?

Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are valid for 6 months from the date of receipt. 

Go To Top Version: 1    Revision Date: 12.14.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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