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Human TruStain FcX™ (Fc Receptor Blocking Solution)

Pricing & Availability
Regulatory Status
RUO
Other Names
Fc Blocker, FcγR blocker, Fc inhibitor
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A_Human_TruStain_FcX_031110
Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant mouse IgG2a PE mAb (above). Dotted histogram represents unstained cells.
  • A_Human_TruStain_FcX_031110
    Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant mouse IgG2a PE mAb (above). Dotted histogram represents unstained cells.
  • A_Human_TruStain_FcX_031110
    Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant rat IgG2b PE mAb (above). Dotted histogram represents unstained cells.
  • C_Human_TruStain_FcX_031110
    Human TruStain FcX™ treated (filled histograms) or non-treated (open histograms) THP-1 cells stained with anti-human HLA-DR PE (red) or an isotype control (IgG2a PE, blue) mAbs. Note: non-treated cells show false-negative HLA-DR staining due to high binding of mouse IgG2a isotype.
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422301 50 tests 101 CHF
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422302 200 tests 229 CHF
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Description

Human Fc receptors (FcRs) are expressed on a variety of cells, such as monocytes, granulocytes, B cells and dendritic cells. The cells with FcR expression sometimes give false positive or false negative results of immunofluorescent staining due to the FcRs-mediated Ig Fc binding. Human TruStain FcX™ is specially formulated for blocking the FcR-involved unwanted staining without interfering with antibody-mediated specific staining of human cells. Human TruStain FcX™ is compatible with flow cytometric staining with anti-human CD16 (clone 3G8), CD32 (clone FUN-2), and CD64 (clone 10.1) antibodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
ICFC, ICC - Reported in the literature, not verified in house
Recommended Usage

It is recommended to add 5 µl of Human TruStain FcX™ per million cells in 100 µl staining volume, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.

Application Notes

This buffer contains specialized human IgG in PBS containing 0.09% sodium azide. It is not recommended to be used for staining human IgG. Handle as biohazard agent.

Application References

(PubMed link indicates BioLegend citation)
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RRID
AB_2818986 (BioLegend Cat. No. 422302)

Antigen Details

Molecular Family
Fc Receptors
Gene ID
NA

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

What is the concentration of Human TruStain FcX™ (Fc receptor blocking solution, Cat#422301)?
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