FITC anti-human CD20 Antibody

Pricing & Availability
Clone
S18015E (See other available formats)
Regulatory Status
RUO
Other Names
B1, Bp35
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S18015E_FITC_CD20_Antibody_030921.png
Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet™ 421 and anti-human CD20 (clone S18015E) FITC (left) or mouse IgG2a, k FITC isotype control (right).
  • S18015E_FITC_CD20_Antibody_030921.png
    Human peripheral blood lymphocytes were stained with anti-human CD19 Brilliant Violet™ 421 and anti-human CD20 (clone S18015E) FITC (left) or mouse IgG2a, k FITC isotype control (right).
See FITC spectral data
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375507 25 tests 45,00 CHF
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375508 100 tests 115,00 CHF
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Description

CD20 (also known as B1 and Bp35) is a four transmembrane spanning protein measuring 33-37 kD. Expressed on pre-B cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. Involved in B-cell activation and is associated with a variety of Src family kinases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD20 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
RRID
AB_2890822 (BioLegend Cat. No. 375507)
AB_2890822 (BioLegend Cat. No. 375508)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Interaction
The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn).
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Hultin LE, et al. 1993. Cytometry 14:196.
  2. Tedder T and Engel P. 1994. Immunol. Today 15:450.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.09.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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