ELISA MAX™ Deluxe Set Mouse G-CSF

Pricing & Availability
Regulatory Status
RUO
Other Names
Granulocyte colony stimulating factor, CSF-3, CSFG, MGI-IG
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ELISA-MAX_Deluxe_Mouse-G-CSF_041723
  • ELISA-MAX_Deluxe_Mouse-G-CSF_041723
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441704 5 plates 360 CHF
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Description

Mouse Granulocyte-Colony Stimulating Factor (G-CSF), also known as mouse CSF-3, is a growth factor and pleiotropic cytokine that influences cells from the bone marrow to differentiate, proliferate and activate the neutrophilic granulocyte lineage (Neutrophils). Mouse G-CSF is produced primarily by monocytes and macrophages upon activation and is measured and tested for differentiation studies. Mouse G-CSF belongs to the IL-6 superfamily and is a polypeptide chain containing 179 amino acids and has a molecular weight of 19 kD. It is naturally produced by the body and is also available as an injection. Human and mouse G-CSF proteins share 76% sequence identity at the amino acid level. 

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse G-CSF Capture Antibody (200X)
  • Mouse G-CSF Detection Antibody (200X)
  • Mouse G-CSF Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)
  • Blocking Buffer A (2X)

Product Details

Verified Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
3.8 - 8.6 pg/mL
Standard Range
12.5 - 800 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water 
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
NA
UniProt
View information about G-CSF on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 04.17.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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