ELISA MAX™ Deluxe Set Human ST2

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin-1 receptor-like 1, ST2, IL1RL1
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ELISA-MAX_Human-ST2_ELISA-KIT_102623
  • ELISA-MAX_Human-ST2_ELISA-KIT_102623
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445004 5 plates 395 CHF
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Description

Il1rl1 (also known as ST2) is a member of the IL-1 superfamily, and its only known ligand is IL-33. ST2 exists in two forms as splice variants. The first form is a soluble form (sST2), which acts as a decoy receptor, sequesters free IL-33, and does not signal. The other form is a membrane-bound form (ST2), which activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 levels are increased in patients with intestinal disease, pulmonary disease, heart disease and various other inflammatory diseases.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human ST2 ELISA MAX™ Capture Antibody (200X)
  • Human ST2 ELISA MAX™ Detection Antibody (200X)
  • Human ST2 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer B (5X)
  • Blocking Buffer A (2X)
  • Assay Diluent B (5X)
  • Assay Diluent C
  • Assay Diluent E 

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
The minimum detectable concentration of Human ST2 for this set in ADC is 45.14 pg/mL and the minimum detectable concentration of Human ST2 for this set in ADE is 48.75 pg/mL
Standard Range
109.4 - 7000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended 
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20 
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended 
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4 , 0.2 g KH2PO4 , 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered 
  • Deionized (DI) water 
  • A microplate reader capable of measuring absorbance at 450 nm 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL 
  • Wash bottle or automated microplate washer 
  • Log-Log graph paper or software for data analysis 
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
B cells, Basophils, Dopaminergic Neurons, Endothelial cells, Eosinophils, Epithelial cells, Lymphocytes, Macrophages, Mast cells, Neutrophils, NK cells, NKT cells, T cells, Th17, Th2, Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines, Soluble Receptors
Gene ID
NA
UniProt
View information about ST2 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 10.26.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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