Brilliant Violet 421™ anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X54-5.7.1_BV421_CD64_Antibody_FC_1_102813.jpg
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on myeloid cell population.
  • X54-5.7.1_BV421_CD64_Antibody_FC_1_102813.jpg
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on myeloid cell population.
  • X54-5.7.1_BV421_CD64_Antibody_FC_2_102813.jpg
Compare all formats See Brilliant Violet 421™ spectral data
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139309 50 µg 292 CHF
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  2. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  3. Gravina A, et al. 2023. Nat Biotechnol. 41:717. PubMed
  4. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  5. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  6. De C, et al. 2023. JCI Insight. 8:. PubMed
  7. Cansever D, et al. 2023. Nat Immunol. . PubMed
  8. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  9. Fontana MF, et al. 2023. Elife. 12: . PubMed
  10. Liu J, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.139. PubMed
  11. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  12. Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
  13. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  14. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  15. Liu J, et al. 2020. Am J Pathol. 190:1298. PubMed
  16. Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed
  17. Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed
  18. Kedage V, et al. 2022. MAbs. 14:2040083. PubMed
  19. Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
  20. Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed
  21. Matheis F, et al. 2020. Cell. 180(1):64-78.e16.. PubMed
  22. Schippers A, et al. 2015. Mucosal Immunol. 101038/mi201582. PubMed
  23. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  24. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  25. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  26. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  27. Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed
  28. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  29. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  30. Draheim M, et al. 2017. EMBO Mol Med. 1.489583333. PubMed
  31. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  32. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  33. Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed
  34. Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
  35. Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
  36. Liu J, et al. 2018. Am J Pathol. 188:2786. PubMed
  37. Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
  38. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  39. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  40. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  41. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  42. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  43. Barnette DN, et al. 2018. JCI Insight. 3. PubMed
  44. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
  45. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  46. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  47. Jones GS, et al. 2020. mSphere. 5:. PubMed
  48. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  49. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  50. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
  51. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
  52. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
RRID
AB_2562694 (BioLegend Cat. No. 139309)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 12.14.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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