Brilliant Violet 421™ anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MEL-14_BV421_032911
C57BL/6 mouse bone marrow cells were stained with CD62L (clone MEL-14) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on total cell population.
  • MEL-14_BV421_032911
    C57BL/6 mouse bone marrow cells were stained with CD62L (clone MEL-14) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on total cell population.
Compare all formats See Brilliant Violet 421™ spectral data
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104435 125 µL 176 CHF
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104436 50 µg 257 CHF
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Valanparambil RM, et al. 2017. PLoS Pathog. 13:e1006647. PubMed
  2. Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed
  3. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  4. Skinner DD, et al. 2022. Front Immunol. 13:931388. PubMed
  5. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  6. Limon JJ et al. 2019. Cell host & microbe. 25(3):377-388 . PubMed
  7. Isakova-Sivak I, et al. 2020. Vaccines (Basel). 8:00. PubMed
  8. Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  9. Li Z, et al. 2022. Elife. 11:. PubMed
  10. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  11. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  12. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  13. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  14. Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
  15. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  16. Kemp V, et al. 2018. Cancer Gene Ther. 26:268. PubMed
  17. Goh PK, et al. 2022. Sci Adv. 8:eabk3338. PubMed
  18. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  19. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  20. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  21. Yue X, et al. 2021. EMBO Rep. 22:e52716. PubMed
  22. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  23. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  24. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  25. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  26. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  27. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  28. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  29. Culina S, et al. 2015. Diabetes. 64: 3532 - 3542. PubMed
  30. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
RRID
AB_10900082 (BioLegend Cat. No. 104435)
AB_2562560 (BioLegend Cat. No. 104436)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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