Brilliant Violet 421™ anti-human CD11a/CD18 (LFA-1) Antibody

Pricing & Availability
Clone
m24 (See other available formats)
Other Names
Integrin β2 subunit, LFA -1 β subunit, β2 integrin, b2 subunit, LFA-1b, LFA-1 beta, LFA1b, LFA1 beta, beta 2 integrin subunit LFA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M24_BV421_CD11aCD18_Antibody_083117
PMA-stimulated human peripheral blood granulocytes were stained with CD11/CD18 (clone m24) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • M24_BV421_CD11aCD18_Antibody_083117
    PMA-stimulated human peripheral blood granulocytes were stained with CD11/CD18 (clone m24) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
363407 25 tests 170 CHF
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363408 100 tests 360 CHF
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Description

CD11/CD18 belongs to the integrin family of proteins. It is heterodimeric cell surface receptor expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocyte adhesion. CD11/CD18 complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. Clone m24 binds the extended/open high affinity conformation of CD11a/CD18. The antibody can be used as a reporter of the activation state of the integrin receptor in response to exposure to Mg2+ or Mn2+.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fibronectin-purified human monocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone m24 can be used as a reporter of the activation state of integrin receptor in response to exposure with Mg2+ or Mn2+.

Application References

(PubMed link indicates BioLegend citation)
  1. Hogg N. et al. 1985. Cell Immunol. 92:247.
  2. McDowall A. et al. 2003. J. Clin. Invest. 111:51.
RRID
AB_2716067 (BioLegend Cat. No. 363407)
AB_2716068 (BioLegend Cat. No. 363408)

Antigen Details

Structure
Integrin family, 95KD, Ig superfamily
Distribution

All leukocytes

Function
Adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type
Dendritic cells, Leukocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301.

Gene ID
16408 View all products for this Gene ID 16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 08.31.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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