APC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_APC_082509
C57BL/6 bone marrow cells stained with HK1.4 APC
  • HK1dot4_APC_082509
    C57BL/6 bone marrow cells stained with HK1.4 APC
See APC spectral data
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128015 25 µg 105,00 CHF
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128016 100 µg 260,00 CHF
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Clemente C, et al. 2018. Nat Commun. 9:910. PubMed
  2. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  3. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  4. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  5. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  6. Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed
  7. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  8. Parlet CP, et al. 2019. Cell Rep. 27:187. PubMed
  9. Ye Z, et al. 2019. Mediators Inflamm. 2019:8709583. PubMed
  10. McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
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  12. MohanKumar K, et al. 2019. Nat Commun. 10:3494. PubMed
  13. Borggrewe M, et al. 2018. Glia. 66:2645. PubMed
  14. Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
  15. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  16. Lubkin A et al. 2019. Cell host & microbe. 25(3):463-470 . PubMed
  17. Green DP, et al. 2019. Neuron. 101:412. PubMed
  18. Ohgomori T et al. 2019. J Comp Neurol. 528(1):14-31 . PubMed
  19. Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
  20. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  21. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  22. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  23. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
  24. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  25. Anderson MC, et al. 2018. Cell Chem Biol. 25:483. PubMed
  26. Ghosh S, et al. 2019. Commun Biol. 0.325. PubMed
  27. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  28. Deczkowska A, et al. 2017. Nat Commun. 8:717. PubMed
  29. Pundir P, et al. 2020. Cell Host & Microbe. 26(1):114-122. PubMed
  30. Werneburg S, et al. 2020. Immunity. 52(1):167-182.e7.. PubMed
  31. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  32. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  33. Li Y, et al. 2020. Theranostics. 10:11376. PubMed
  34. Getts D, et al. 2014. Sci Transl Med. 15:219. PubMed
  35. Ray A, et al. 2014. J Immunol. 192:5109. PubMed
  36. Collins C, et al. 2014. Proc Natl Acad Sci U S A. 111:9899. PubMed
  37. Martin R, et al. 2014. J Leukoc Biol. 96:151. PubMed
  38. Pisano F, et al. 2014. PLoS One. 9:103541. PubMed
  39. Sauter K, et al. 2014. J Leukoc Biol. 96:265. PubMed
  40. Price P, et al. 2014. J Virol. 88:10840. PubMed
  41. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  42. Tebartz C, et al. 2015. J Immunol. 194:1100. PubMed
  43. Tanaka T, et al. 2015. J Immunol. 194:2004. PubMed
  44. Rosenheinrich M, et al. 2015. PLoS One. 10: 0136290. PubMed
  45. Kratochvill F, et al. 2015. Cancer Res. 75: 3054-3064. PubMed
  46. Mukovozov I, et al. 2015. J Immunol. 195: 3334 - 3344. PubMed
  47. Radjabova V, et al. 2015. J Immunol. 195: 3149 - 3159. PubMed
  48. Nocera D, et al. 2016. J Immunol. 196: 2860 - 2869. PubMed
  49. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  50. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  51. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  52. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
  53. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  54. Karki R, et al. 2021. Cell. 184:149. PubMed
  55. Ritzel RM, et al. 2021. Glia. 69:746. PubMed
  56. Bordas M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  57. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  58. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  59. Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed
  60. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  61. Ohgomori T, et al. 2020. Neuropharmacology. 176:108227. PubMed
  62. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  63. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  64. Shao M, et al. 2021. Cell Stem Cell. 28(4):685-701.e7. PubMed
  65. Joffin N, et al. 2021. Cell Stem Cell. 28(4):702-717.e8. PubMed
  66. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  67. Pingili AK, et al. 2021. Cell Reports. 35(12):109285. PubMed
RRID
AB_1732087 (BioLegend Cat. No. 128015)
AB_1732076 (BioLegend Cat. No. 128016)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.09.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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