APC anti-human CD62E Antibody

Pricing & Availability
Clone
HAE-1f (See other available formats)
Other Names
E-selectin, ELAM-1, LECAM-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HAE-1f_APC_021009.jpg
TNF-α-stimulated (6hr) HUVEC cells stained with CD62E (clone HAE-1f) APC
  • HAE-1f_APC_021009.jpg
    TNF-α-stimulated (6hr) HUVEC cells stained with CD62E (clone HAE-1f) APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
336011 25 tests 120 CHF
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336012 100 tests 265 CHF
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Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162).The HAE-1f antibody has been shown to recognize human CD62E and to be useful for flow cytometry and in vitro blocking.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications: blocks CD62E binding to its ligand

Application References

(PubMed link indicates BioLegend citation)
  1. Tu L, et al. 1996. J. Immunol. 157:3995
  2. Zhang Y and S. Neelamegham. 2002. Biophys. J. 83:1934
Product Citations
  1. Jeffery H, et al. 2013. J Virol. 87:6530. PubMed
  2. Tzoran I, et al. 2015. Exp Hematol. . PubMed
  3. Hijmans JG, et al. 2019. J Am Heart Assoc. 8:e011134. PubMed
Publication Library
RRID
AB_2254490 (BioLegend Cat. No. 336011)
AB_2186829 (BioLegend Cat. No. 336012)

Antigen Details

Structure
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution

Highly expressed on activated endothelial cells (IL-2, TNF-α), other cytokines can increase expression); can also be expressed on endothelial cells in the skin, bone marrow and placenta.

Function
Involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor
Siayl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported in interact with CD62E
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Collins T, et al. 1991. J. Biol. Chem. 266:2466
2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Lawrence MB, et al. 1993. J. Immunol. 151:6338.
5. Walz G, et al. 1990. Science 250:1132

Gene ID
6401 View all products for this Gene ID
UniProt
View information about CD62E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.20.2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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