APC anti-human CD326 (EpCAM) Antibody

Pricing & Availability
Clone
9C4 (See other available formats)
Regulatory Status
RUO
Other Names
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
9C4_APC_020708
Human colon carcinoma cell line HT29 stained with 9C4 APC
  • 9C4_APC_020708
    Human colon carcinoma cell line HT29 stained with 9C4 APC
Compare all formats See APC spectral data
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324207 25 tests 149 CHF
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324208 100 tests 314 CHF
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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DU.4475 breast carcinoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Lammers R, et al. 2002. Exp. Hematol. 30:537.
  2. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
  3. Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Tang C, et al. 2020. Stem Cell Research. 39:101511.. PubMed
  2. Yamaguchi N, et al. 2020. eLife. 8:e52135.. PubMed
  3. Mulay A, et al. 2021. Cell Reports. 35(5):109055. PubMed
  4. Yu XX, et al. 2021. Cell Res. 31:886. PubMed
  5. Segal JM, et al. 2019. Nat Commun. 10:3350. PubMed
  6. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  7. Puca L, et al. 2018. Nat Commun. 9:2404. PubMed
  8. Yamashiro C, et al. 2020. Nat Protoc. 15:1560. PubMed
  9. Apostolou P, Ntanovasilis D, and Papasotiriou I. 2017. J Biol Res (Thessalon). 10.1186/s40709-017-0068-9. PubMed
  10. Mota STS, et al. 2019. Int J Mol Sci. 20. PubMed
  11. Go YH, et al. 2022. Cancers (Basel). 14:. PubMed
  12. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  13. Marquez-Curtis LA, et al. 2022. PLoS One. 17:e0263005. PubMed
  14. Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
  15. Haraguchi T, et al. 2016. Sci Rep. 6:21117. PubMed
  16. Koh SB, et al. 2021. NPJ Precis Oncol. 5:85. PubMed
  17. Skrbo N, et al. 2014. PLoS One. 9:113278. PubMed
  18. Chua CW, et al. 2018. Elife. 7. PubMed
  19. Lo PK, et al. 2018. Elife. 7:e31334. PubMed
  20. Liu L, et al. 2018. Clin Cancer Res. 24:2370. PubMed
  21. Fujii M, et al. 2018. Cell Stem Cell. 23:787. PubMed
  22. Hornburg M, et al. 2021. Cancer Cell. . PubMed
  23. Yoshida K, et al. 2020. Nature. 578:266. PubMed
  24. Murase Y, et al. 2020. EMBO J. 39:e104929. PubMed
  25. Huerta–Nuñ ez LFE, et al. 2019. Sci Rep. 9:6419. PubMed
  26. Chen D, et al. 2019. Cell Rep. 29:4568. PubMed
  27. Balakrishnan A, et al. 2019. Clin Cancer Res. 25:7506. PubMed
  28. Dhanwani R, et al. 2020. Sci Adv. 6:eaba3688. PubMed
  29. Hancock GV, et al. 2021. Stem Cell Res. 55:102493. PubMed
  30. Enge M, et al. 2017. Cell. 171:321. PubMed
  31. Yamashiro C, et al. 2020. Nature Protocols. 362(6412):356-360. PubMed
  32. Mou H, et al. 2021. Cell Reports. 35(3):109011. PubMed
  33. Chen D et al. 2018. Cell reports. 25(13):3591-3602 . PubMed
  34. Grant RA, et al. 2021. Nature. 590(7847):635-641. PubMed
  35. Nian Z, et al. 2021. Clin Cancer Res. 27:6026. PubMed
  36. Xiang X, et al. 2022. Nat Commun. 13:463. PubMed
  37. Mithal A, et al. 2020. Nat Commun. 0.607638889. PubMed
  38. Wosen JE, et al. 2019. Front Immunol. 1.784722222. PubMed
  39. Kojima Y et al. 2017. Cell stem cell. 21(4):517-532 . PubMed
  40. Chen D, et al. 2017. Biol Reprod. 97:850. PubMed
  41. Yan X, et al. 2019. Oncol Rep. 41:1938. PubMed
  42. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  43. Yuan W, et al. 2021. Int J Biol Sci. 17:527. PubMed
  44. Monterosso ME, et al. 2021. Sci Rep. 5118:11. PubMed
  45. Nelson L, et al. 2015. Sci Rep. 5: 17455. PubMed
  46. Menck K, et al. 2017. J Extracell Vesicles. 10.1080/20013078.2017.1340745. PubMed
  47. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
  48. Ludwig LS et al. 2019. Cell. 176(6):1325-1339 . PubMed
RRID
AB_756081 (BioLegend Cat. No. 324207)
AB_756082 (BioLegend Cat. No. 324208)

Antigen Details

Structure
Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification
Glycosylated.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.

Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD326 (EpCAM) 9C4 FC,ICC,IHC
FITC anti-human CD326 (EpCAM) 9C4 FC
PE anti-human CD326 (EpCAM) 9C4 FC
APC anti-human CD326 (EpCAM) 9C4 FC
Alexa Fluor® 488 anti-human CD326 (EpCAM) 9C4 FC,SB
Alexa Fluor® 647 anti-human CD326 (EpCAM) 9C4 FC,ICC,SB
PerCP/Cyanine5.5 anti-human CD326 (EpCAM) 9C4 FC
Biotin anti-human CD326 (EpCAM) 9C4 FC
Pacific Blue™ anti-human CD326 (EpCAM) 9C4 FC
Brilliant Violet 421™ anti-human CD326 (EpCAM) 9C4 FC
PE/Cyanine7 anti-human CD326 (EpCAM) 9C4 FC
Brilliant Violet 605™ anti-human CD326 (EpCAM) 9C4 FC
Brilliant Violet 650™ anti-human CD326 (EpCAM) 9C4 FC
Alexa Fluor® 594 anti-human CD326 (EpCAM) 9C4 IHC-P,ICC,SB
Purified anti-human CD326 (EpCAM) (Maxpar® Ready) 9C4 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD326 (EpCAM) 9C4 FC
APC/Fire™ 750 anti-human CD326 (EpCAM) 9C4 FC
Brilliant Violet 510™ anti-human CD326 (EpCAM) 9C4 FC
Brilliant Violet 785™ anti-human CD326 (EpCAM) 9C4 FC
Brilliant Violet 711™ anti-human CD326 (Ep-CAM) 9C4 FC
TotalSeq™-A0123 anti-human CD326 (Ep-CAM) 9C4 PG
APC/Cyanine7 anti-human CD326 (Ep-CAM) 9C4 FC
Alexa Fluor® 700 anti-human CD326 (EpCAM) 9C4 FC
TotalSeq™-C0123 anti-human CD326 (Ep-CAM) 9C4 PG
TotalSeq™-B0123 anti-human CD326 (Ep-CAM) 9C4 PG
PE/Cyanine5 anti-human CD326 (Ep-CAM) 9C4 FC
TotalSeq™-D0123 anti-human CD326 (Ep-CAM) 9C4 PG
Spark UV™ 387 anti-human CD326 (Ep-CAM) 9C4 FC
Spark Red™ 718 anti-human CD326 (Ep-CAM) (Flexi-Fluor™) 9C4 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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