Anti-Synaptobrevin (VAMP) Antibody (Previously Covance catalog# MMS-616R)

Pricing & Availability
Clone
SP10 (See other available formats)
Regulatory Status
RUO
Other Names
Vesicle-associated membrane protein 1, synaptobrevin-1
Previously
Covance Catalog# MMS-616R
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Sp10_Ascites_Synaptobrevin_Antibody_021919_updated.png
Western blot of anti-Synaptobrevin (VAMP) antibody (clone SP10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with a 1:2000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
  • Sp10_Ascites_Synaptobrevin_Antibody_021919_updated.png
    Western blot of anti-Synaptobrevin (VAMP) antibody (clone SP10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with a 1:2000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
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837001 500 µL 372,00 CHF
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Description

Synaptobrevins, also known as VAMPs, along with syntaxins and the synaptosomal-associated protein SNAP25, form the protein complex that is involved with the docking and fusion of synaptic vesicles with the presynaptic membrane. Synaptobrevins have a molecular weight of 18 kD, and are also involved in the formation of the SNARE complexes. The synaptobrevins are degraded by the protein tetanospasmin, from the tetanus-causing bacterium Clostridium tetani, as well as botulinum toxin from Clostridium botulinum.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a crude synaptic preparation of post mortem human brain.
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB - Quality tested
IHC-P, ELISA, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1000 - 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SP10 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118) and VAMP-2 expressed in a bacterial system (aa 33-96). The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections. 

Application References

(PubMed link indicates BioLegend citation)
  1. Sawada K, et al. 2002. Mol Psychiatry 7:484-92. (WB, IHC-P, ELISA)
  2. Honer WG, et al. 2002. Cerebral Cortex 12:349-56. (WB, ELISA, ICC)
  3. Honer WG, et al. 1993. Brain Res. 609:9-20. (ICC)
  4. Poon VY, et al. 2014. Front. Cell Neurosci. 8:66. (ICC) PubMed
Product Citations
  1. Poon V, et al. 2014. Front Cell Neurosci. 8:66. PubMed
RRID
AB_2565369 (BioLegend Cat. No. 837001)

Antigen Details

Structure
Synaptobrevin 1 (VAMP1) is a 118 amino acid protein with an apparent molecular mass of 13kD. Synaptobrevin 2 (VAMP2) is a 116 amino acid protein with an apparent molecular mass of 12.5 kD.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6843 View all products for this Gene ID
UniProt
View information about Synaptobrevin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10.29.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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