Alexa Fluor® 647 anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
N418_A647_CD11c_Antibody_1_HR_090717
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647
  • N418_A647_CD11c_Antibody_1_HR_090717
    C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647
  • N418_A647_CD11c_Antibody_3_HR_090717
    Fixed whole mount mouse epidermis Langerhans cells were stained with Alexa Fluor® 647 CD11c (red) (clone N418) and PE CD207 (yellow) (clone 4C7). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
  • N418_A647_CD11c_Antibody_4_HR_090717
    Live intravital mouse spleen imaging. PE CD11b (red) (clone M1/70), Alexa Fluor® 488 F4/80 (green) (clone BM8), and Alexa Fluor® 647 CD11c (blue) (clone N418) were imaged 30 minutes after iv injection of 10 µg per antibody. Isotype controls at the same concentrations, time post injection, and exposure conditions were used for the negative control. Cells were imaged with a Leica SP5 confocal on anesthetized mice. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
  • N418_A647_CD11c_Antibody_6_HR_090717
    Fixed whole mount mouse spleen was stained with FITC CD172a (red) (clone P84), Alexa Fluor® CD11c (yellow) (clone N418), and PE CD11b (blue) (clone M1/70). Isotype controls at the same concentrations were used for the negative control. Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
  • N418_AF647_CD11c_Antibody_7_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD8a Antibody (clone 53-6.7) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD11c Antibody (clone N418) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 647 spectral data
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117314 25 µg 85,00 CHF
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117312 100 µg 195,00 CHF
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified
IHC - Reported but collaborator, not verified in house
ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
Product Citations
  1. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  2. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  3. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  4. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  5. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  6. Markey KA et al. 2019. Current protocols in immunology. 125(1):e72 . PubMed
  7. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  8. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  9. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  10. Bradford BM, et al. 2018. Parasite Immunol. 40:e12566. PubMed
  11. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  12. An S, et al. 2019. Small. 15:e1805182. PubMed
  13. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  14. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  15. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  16. Bates J, et al. 2009. J Immunol. 182:7539. PubMed
  17. Chen S, et al. 2015. Blood. 126: 103 - 112. PubMed
  18. Lei G, et al. 2016. Antimicrob Agents Chemother. 60: 1289 - 1297. PubMed
  19. Reboldi A, et al. 2016. Sci Transl Med. 352: aaf4822. PubMed
  20. Collins N, et al. 2016. Nat Commun. 7:11514. PubMed
  21. Mambres D, et al. 2016. J Immunol. 196: 3780 - 3793. PubMed
  22. Fox B, et al. 2016. PLoS One. 12: 1006189. PubMed
  23. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  24. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  25. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  26. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  27. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  28. Koikawa K, et al. 2021. Cell. 184(18):4753-4771.e27. PubMed
RRID
AB_492850 (BioLegend Cat. No. 117314)
AB_389328 (BioLegend Cat. No. 117312)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 09.21.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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