Alexa Fluor® 594 anti-human CD133 Antibody

Pricing & Availability
Clone
clone 7 (See other available formats)
Other Names
Prominin-1, AC133 Antigen, PROM1, PROML1
Isotype
Mouse IgG1, κ
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Product Citations
publications
clone7_A594_CD133_Antibody_HR_092717
Human paraffin-embedded small intestine tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris Buffered 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human CD133 (clone clone 7, red) and Alexa Fluor® 647 anti-human Vimentin antibody (clone O91D3, green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • clone7_A594_CD133_Antibody_HR_092717
    Human paraffin-embedded small intestine tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris Buffered 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human CD133 (clone clone 7, red) and Alexa Fluor® 647 anti-human Vimentin antibody (clone O91D3, green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
372813 25 µg 135 CHF
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372814 100 µg 325 CHF
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Description

CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant partial human CD133 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry staining. For immunohistochemistry, a concentration range of 2.5-10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This clone can block the staining of AC133 clone in flow cytometry.

Application References

(PubMed link indicates BioLegend citation)
  1. Swaminathan SK, et al. 2010. J. Immunol. Methods 361:110.
RRID
AB_2716226 (BioLegend Cat. No. 372813)
AB_2716227 (BioLegend Cat. No. 372814)

Antigen Details

Structure
120 kD pentaspan transmembrane glycoprotein.
Distribution

Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells

Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References
  1. Yin AH, et al. 1997. Blood. 90:5002.
  2. Miraglia S, et al. 1997. Blood. 90:5013.
  3. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25. 
Gene ID
8842 View all products for this Gene ID
UniProt
View information about CD133 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 09.10.2019

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