Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2758 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
400525 100 µg 165 CHF
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Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimoto H, et al. 2005. Blood 106:4241.
  2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
  3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations
  1. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  2. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  3. Pelon F, et al. 2020. Nat Commun. 0.738888889. PubMed
  4. Davis F, et al. 2015. Proc Natl Acad Sci U S A. 112:5827. PubMed
  5. Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed
  6. Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed
  7. Ojeda-Fernández L, et al. 2016. PLoS One. 12: 1005935. PubMed
  8. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  9. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  10. Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed
  11. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
RRID
AB_2864283 (BioLegend Cat. No. 400525)

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Biotin Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
FITC Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PE Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC, WB, IP, ICC, IHC, FA, ChIP
PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Pacific Blue™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PerCP Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC, WB, IP, ICC, IHC, FA
Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl RTK2758 ICC
GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC, WB, IP, ICC, IHC, FA
APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Go-ChIP-Grade™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 ChIP, FC
TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Go To Top Version: 2    Revision Date: 06.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2a, κ Isotype Ctrl

  • Biotin Rat IgG2a, κ Isotype Ctrl

  • FITC Rat IgG2a, κ Isotype Ctrl

  • PE Rat IgG2a, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl

  • Purified Rat IgG2a, κ Isotype Ctrl

  • PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl

  • PerCP Rat IgG2a, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl

  • Go-ChIP-Grade™ Purified Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl

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