- Clone
- 561 (See other available formats)
- Other Names
- Gp105-120, My10, Mucosialin
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells stained with CD45 APC and CD34 (clone 561) Alexa Fluor® 488 (top) or Alexa Fluor® 488 mouse IgG2a, κ isotype control (bottom). Data shown was gated on CD14- cell population. -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 343619 | 25 tests | 105 CHF | ||||
| 343620 | 100 tests | 225 CHF | ||||
CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
Product DetailsProduct Details
- Reactivity
- Human, Cross-Reactivity: Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
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The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.
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Application References
(PubMed link indicates BioLegend citation) -
- Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82.
- Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
- RRID
-
AB_2632720 (BioLegend Cat. No. 343619)
AB_2632721 (BioLegend Cat. No. 343620)
Antigen Details
- Structure
- 105-120 kD single chain mucin-like glycoprotein
- Distribution
- Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
- Function
- Cell adhesion
- Ligand/Receptor
- L-selecin, E-selectin
- Cell Type
- Hematopoietic stem and progenitors, Endothelial cells, Fibroblasts
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- CD Molecules
- Gene ID
- 947 View all products for this Gene ID
- UniProt
- View information about CD34 on UniProt.org
Related Pages & Pathways
Pathways
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