While traditional flow cytometry allows for rapid, multi-dimensional characterization of cell populations, it lacks the resolution and capacity to perform simultaneous proteomic and genomic analysis on a single-cell scale. BioLegend now offers oligonucleotide-conjugated TotalSeq™ antibodies designed for single-cell proteogenomic analysis via cellular indexing of transcriptomes and epitopes by sequencing, or CITE-Seq. They are also compatible with similar workflows, such as REAP-seq.
In place of fluorophores, each TotalSeq™ clone is conjugated to an oligonucleotide that includes a unique 15 nucleotide (nt) barcode, which converts antigen signal into a readable sequence. Combining antigen detection with single cell RNA sequencing (scRNA-Seq) allows for simultaneous transcriptomic and proteomic analyses in individual cells. Furthermore, since fluorophore and detector availabilities are no longer a factor, a significantly larger number of markers that can be analyzed at once. So far, simultaneous analysis of over 80 surface markers with scRNA-Seq has been reported in the literature.
In addition to unique antigen-specific antibodies, we also carry oligo-conjugated cell hashing reagents, which contain antibodies to ubiquitously expressed cell markers to facilitate sample multiplexing and doublet exclusion. TotalSeq™ antibodies are readily compatible with 10X Genomics scRNA-Seq and Illumina sequencing platforms, but are also adaptable to other droplet-based single-cell workflows with assay optimization.