Biotin anti-mouse CD229 (Ly-9) Antibody

Pricing & Availability
Clone
Ly9ab3 (See other available formats)
Regulatory Status
RUO
Other Names
Ly9, SLAMF3
Isotype
Armenian Hamster IgG
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Product Citations
publications
Ly9ab3_Biotin_090707
C57BL/6 mouse splenocytes stained with biotinylated Ly9ab3, followed by Sav-PE
  • Ly9ab3_Biotin_090707
    C57BL/6 mouse splenocytes stained with biotinylated Ly9ab3, followed by Sav-PE
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122903 50 µg 67€
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Description

CD229 is a 100-120 kD glycoprotein. It is a member of the SLAM family, a CD2 subset of the Ig superfamily, known as Ly9 or SLAMF3. CD229 is expressed on T cells, B cells, NK cells, and thymocytes. It functions as a homophilic adhesion molecule through binding to CD229 itself. The cytoplasmic tail of CD229 binds to SAP and Grb2 proteins. CD229 is involved in enhancing T cell activation and Th2 polarization.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
AHK cells transiently transfected with mouse CD229 (Ly-9)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Romero X, et al. 2005. J. Immunol. 174:7033.
Product Citations
  1. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
RRID
AB_830724 (BioLegend Cat. No. 122903)

Antigen Details

Structure
100-120 kD glycoprotein, SLAM family, CD2 subset of Ig superfamily
Distribution

T cells, B cells, NK cells, thymocytes

Function
Homophilic adhesion, T cell activation, Th2 cell polarization
Ligand/Receptor
CD229
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sandrin MS, et al. 1996. Immunogenetics 43:13.
2. de la Fuente MA, et al. 2001. Blood 97:3513.
3. Romero X, et al. 2005. J. Immunol. 174:7033.
4. Martin M, et al. 2005. J. Immunol. 174:5977.
5. Graham DB, et al. 2006. J. Immunol. 176:291.

Gene ID
17085 View all products for this Gene ID
UniProt
View information about CD229 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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