Purified anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
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Product Citations
publications
1-MEL-14_Purified_090707
C57BL/6 mouse splenocytes stained with purified MEL-14, followed by anti-rat IgG FITC
  • 1-MEL-14_Purified_090707
    C57BL/6 mouse splenocytes stained with purified MEL-14, followed by anti-rat IgG FITC
  • 2-MEL-14_pure_CD62L_Antibody_2_103018
    Fresh, frozen mouse spleen was stained with purified CD62L clone MEL-14 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with Ly6c Cy3 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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104402 500 µg $115.00
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Gil-Pulido J, et al. 2017. PLoS One.. 10.1371/journal.pone.0181947. PubMed
  2. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  3. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  4. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  5. Morabito KM, et al. 2017. Mucosal Immunol. 0.795138889. PubMed
  6. Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed
  7. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  8. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  9. Ban YH, et al. 2017. Cell Rep. 2.6375. PubMed
  10. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  11. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  12. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  13. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  14. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  15. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  16. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  17. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  18. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  19. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  20. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  21. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  22. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  23. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  24. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  25. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  26. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  27. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  28. Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
  29. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  30. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  31. Yi J, et al. 2019. Mol Cells. 42:228. PubMed
  32. Jun JC, et al. 2017. PLoS One. 12:e0180688. PubMed
  33. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  34. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  35. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  36. Richards KA, et al. 2018. Front Immunol. 0.829861111. PubMed
  37. Managlia E, et al. 2019. Am J Pathol. 189:604. PubMed
  38. Olver S, et al. 2005. Cancer Res. 66:571. PubMed
  39. Fukushima A, et al. 2006. Invest Ophthalmol Vis Sci. 47:657. PubMed
  40. Benson M, et al. 2007. J Exp Med. 204:1765. PubMed
  41. Kenna T, et al. 2008. Blood. 111:2091. PubMed
  42. Florek M, et al. 2015. PLoS One. 10: 0145763. PubMed
  43. Lorsung RM, et al. 2020. Front Bioeng Biotechnol. 0.9375. PubMed
  44. Karuppuchamy T, et al. 2020. Inflamm Bowel Dis. 216:26. PubMed
  45. Muhammad F, et al. 2020. Front Immunol. 975:11. PubMed
  46. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
RRID
AB_313089 (BioLegend Cat. No. 104402)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10/30/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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