- M6308B04 (See other available formats)
- Regulatory Status
- Other Names
- Matrix metallopeptidase 2, Gelatinase A, MMP-2, GelA, Clg4a, CLG4, MONA, TBE-1, MMP-II
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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MMP2, also named gelatinase A and MMP-2, is a member of matrix metalloproteinase family of proteins (MMPs). MMPs are structurally related enzymes that contain zinc that degrade the extracellular matrix and connective tissue proteins in normal physiological processes such as embryonic development, reproduction, and tissue remodeling as well as in disease processes such as arthritis and metastasis. MMP2 consists of a prodomain, which is cleaved upon activation, a catalytic domain containing the zinc binding site, a fibronectin-like domain (that plays a role in the substrate targeting), and a carboxyl-terminal (hemopexin-like repeats) domain. The activation of MMP2 requires proteolytic processing starting first with a complex of membrane type 1 MMP (MT1-MMP) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP2 from the extracellular milieu to the cell surface. The second step of the process is MMP2 activating by active MT1-MMP and subsequent autocatalytic cleavage. Substrates of MMP2 include type IV collagen, aggrecan, link protein, decorin, fibronectin, and type X and XI collagens, which all are components of the articular cartilaginous matrix. Importantly, MMP2 secretion is elevated in several types of human cancers and its elevated expression has been associated with a poor prognosis. Mutations in the MMP2 gene are associated with Torg-Winchester syndrome, multicentric osteolysis, arthritis syndrome, and possibly keloids. MMP2 deficient mice exhibit slightly delayed growth, reduced neovascularization, retarded tumor progression, an exaggerated asthma response to allergens, and impaired branching morphogenesis of the mammary gland.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Recombinant human MMP-2, amino acids (Ala30-Cys660) (Accession# NP_004521.1) with a C-terminal His tag and a linker sequence. It is expressed in 293E cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2566323 (BioLegend Cat. No. 680002)
- Monomer. Human MMP2 contains 660 amino acids with a predicted molecular weight of 73.9 kD. Upon activation by p-aminophenylmercuric acetate (APMA), it has a predicted molecular weight of 62 kD.
MMP2 is expressed by fibroblasts, endothelial cells, alveolar epithelial cells, and macrophages.
- MMP2 is a metalloproteinase that specifically cleaves type IV collagen, which is the major structural component of basement membranes. MMP2 is thought to contribute to the invasion and the metastasis of various human malignancies.
- Fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans, decorin, osteopontin, and IBP-3.
- TIMPs, CLL7, and thrombospondin 1.
- Cell Type
- Endothelial cells, Epithelial cells, Fibroblasts, Macrophages
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Enzymes and Regulators
- Antigen References
1. Nagase H and Woessner JF. 1999. J. Biol. Chem. 274:21491.
2. Martignetti JA, et al. 2001. Nat. Genet. 28:261.
3. Itoh T, et al. 1997. J. Biol. Chem. 272:22389.
4. Passlick B, et al. 2000. Clin. Cancer Res. 6:3944.
5. Qian Q, et al. 2010. Cancer Invest. 28:661.
6. Hoikkala S, et al. 2006. Cancer Lett. 236:125.
7. Yamamura T, et al. 2002. Cancer 35:249.
- Gene ID
- 4313 View all products for this Gene ID
- View information about MMP-2 on UniProt.org
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