Purified anti-Lamin A/C Antibody

Pricing & Availability
Clone
Poly6135 (See other available formats)
Regulatory Status
RUO
Other Names
LMNA, LMNC, CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
1_Poly6135_PURE_LaminAslashC_Antibody_1_WB_072921.png
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-Lamin A/C. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
  • 1_Poly6135_PURE_LaminAslashC_Antibody_1_WB_072921.png
    Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-Lamin A/C. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
  • 2_Poly6135_PURE_LaminA_Antibody_2_ICC_080519
    HeLa cells were fixed with 2% PFA for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-Lamin A/C Antibody (clone Poly6135) followed by Alexa Fluor® 594 (red) conjugated goat anti-Rabbit IgG in blocking buffer for two hours at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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613501 50 µl (5 Western blots) $75.00
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613502 200 µl (20 Western blots) $225.00
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Description

Lamins A and C are structural components of the lamina, a scaffold of proteins localized to the nuclear inner membrane, and contribute to nuclear membrane dynamics and chromatin structure. Mutations in the LMNA gene have been linked to multiple laminopathy disorders, including Hutchinson-Gilford progeria syndrome (HGPS), an autosomal dominant disorder characterized by the appearance of accelerated and dramatic aging. Patients with HGPS have a mutation in the LMNA gene that results in aberrant splicing and production of the toxic, truncated Lamin A protein progerin that functions in a dominant negative pattern.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse*
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant (partial), C-terminal
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, the suggested usage is 1.0 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes

*This antibody weakly cross-reacts with Mouse.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Y, et al. 2007. Mol Cancer Res. 5:909. PubMed
  2. Takezaki S, et al. 2012. J. Immunol. 189:1521. PubMed.
Product Citations
  1. Shafiee A,et al. 2017. Sci Rep.. 10.1038/s41598-017-13971-3. PubMed
  2. Mori H, et al. 2018. J Dermatol Sci. 90:154. PubMed
  3. Zhang Y, et al. 2007. Mol Cancer Res. 0.839583333. PubMed
  4. Qian Z, et al. 2010. PLoS One. 6:e1000814. PubMed
  5. Takezaki S, et al. 2012. J Immunol. 189:1521. PubMed
RRID
AB_315796 (BioLegend Cat. No. 613501)
AB_315797 (BioLegend Cat. No. 613502)

Antigen Details

Structure
Intermediate filament family, farnesyl binding domain, dimer. Isoforms A, AD10, C, approximately 70 kD, 66 kD, and 61 kD, respectively
Distribution

Nucleoplasmic side of inner nuclear membrane

Function
Fibrous component of nuclear lamina, provides framework for nuclear envelope, may interact with chromatin
Interaction
Homodimeric with Lamin C, interacts with LAPs 1A-1C, emerin, Narf, hsMOK2, PKC, SREBP1a, SREBP 1c
Modification
Phosphorylation, Methylation, Farnesylation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Antigen References

1. Machiels B, et al. 1996. J. Biol. Chem. 271:9249.
2. Barton R, et al. 1999. J. Biol. Chem. 274:30008.
3. Gruenbaum Y, et al. 2000. J. Struct. Biol. 129:313.
4. Stierle V, et al. 2003. Biochemistry 42:4819.

Regulation
Strictly under cell cycle control as seen by disintegration/formation of nuclear envelope in prophase/telophase. Phosphorylation regulates disassembly
Gene ID
4000 View all products for this Gene ID
UniProt
View information about Lamin A/C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 07/27/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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