PE/Cyanine7 anti-mouse/human CD324 (E-Cadherin) Antibody

Pricing & Availability
Clone
DECMA-1 (See other available formats)
Regulatory Status
RUO
Other Names
E-Cadherin, Cadherin-1, CDH1, and UVO
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
DECMA-1_PECy7_CD324_Antibody_FC_090414.jpg
MDCK epithelial cell line was stained with CD324 (clone DECMA-1) PE/Cyanine7 (filled histogram) or rat IgG1, ? PE/Cyanine7 isotype control (open histogram).
  • DECMA-1_PECy7_CD324_Antibody_FC_090414.jpg
    MDCK epithelial cell line was stained with CD324 (clone DECMA-1) PE/Cyanine7 (filled histogram) or rat IgG1, ? PE/Cyanine7 isotype control (open histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
147309 25 µg $105.00
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147310 100 µg $315.00
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Description

CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Dog (Canine), Swine (Pig, Porcine)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E-Cadherin extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1, Western Blotting1, immunomicroscopy3, and biological function1,2.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
  2. Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
  3. Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)
Product Citations
  1. Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed
  2. Li H, et al. 2019. J Immunol Res. 2018:4807145. PubMed
  3. Hreha TN, et al. 2020. Physiol Rep. 8:e14401. PubMed
  4. Foster DS, et al. 2020. Nat Commun. 3.278472222. PubMed
  5. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  6. Kinoshita M, et al. 2020. Cell Stem Cell. . PubMed
RRID
AB_2564187 (BioLegend Cat. No. 147309)
AB_2564188 (BioLegend Cat. No. 147310)

Antigen Details

Structure
Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution

Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.

Function
Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Interaction
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand/Receptor
αEβ7 integrin.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.

Gene ID
12550 View all products for this Gene ID 999 View all products for this Gene ID
UniProt
View information about CD324 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/16/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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