FITC anti-human MRP-14 (S100A9) Antibody

Pricing & Availability
Clone
MRP 1H9 (See other available formats)
Regulatory Status
RUO
Other Names
S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype
Mouse IgG1, κ
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Product Citations
publications
MRP14_FITC_1_100610
Human peripheral blood granulocytes surface stained with anti-human CD15-PerCP/Cy5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP14-FITC (clone 1H9) (top) or mouse IgG1 FITC isotype control (middle)
  • MRP14_FITC_1_100610
    Human peripheral blood granulocytes surface stained with anti-human CD15-PerCP/Cy5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP14-FITC (clone 1H9) (top) or mouse IgG1 FITC isotype control (middle)
  • MRP14_FITC_2_100610
See FITC spectral data
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350703 25 tests $95.00
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350704 100 tests $215.00
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Description

MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and has been considered an endogenous activator of Toll-like receptor 4. S100A9 has been identified as a marker for CD14(+) HLA-DR(-/low) MDSCs.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MRP-14
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone has been published as 1H9.

Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining  with paraffin-embedded tissue sections1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Robinson MJ, et al. 2002. J. Biol. Chem. 277:3658. (WB, IHC)
  2. Croce K, et al. 2009. Circulation 120:427. (IHC)
  3. Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed
RRID
AB_10662414 (BioLegend Cat. No. 350703)
AB_10662250 (BioLegend Cat. No. 350704)

Antigen Details

Structure
Belongs to S100 family of proteins containing 2-EF-hand calcium -binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8
Distribution

MDSCs, neutrophils, monocyte, and ileal tissue eosinophils

Function
Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Interaction
Calcium
Cell Type
Neutrophils, Monocytes, Eosinophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Adhesion Molecules
Antigen References

1. Loser K, et al. 2010. Nat. Med. 16:713.
2. Cheng P, et al. 2008. J. Exp. Med. 205:2235.
3. Leukert N, et al.2006. J. Mol. Biol. 359:961.
4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402.
5. Zhao F, et al. 2012. Immunology. 136:176. 

Gene ID
6280 View all products for this Gene ID
UniProt
View information about MRP-14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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