Biotin anti-human MMP-2 Antibody

Pricing & Availability
Clone
M6310C11 (See other available formats)
Regulatory Status
RUO
Other Names
Matrix metallopeptidase 2, Gelatinase A, MMP-2, GelA, Clg4a, CLG4, MONA, TBE-1, MMP-II, MMP2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
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532001 100 µg $225.00
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Description

MMP-2, also known as gelatinase A, is a member the of matrix metalloproteinase family of proteins (MMPs). MMPs are structurally-related calcium-dependent, zinc-containing enzymes that degrade extracellular matrix and connective tissue proteins in normal physiological processes such as embryonic development, reproduction, and tissue remodeling. MMPs are also active in disease processes such as arthritis and metastasis.

 

MMP-2 consists of a pro-domain (cleaved upon activation), a catalytic domain containing the zinc binding site, a fibronectin-like domain (that plays a role in the substrate targeting), and a carboxyl-terminal hemopexin-like domain. The activated form of MMP-2 is produced by a 2-step proteolytic processing. First, a complex comprised of membrane type 1 MMP (MT1-MMP) and tissue inhibitor of metalloproteinase 2 (TIMP-2) recruits pro-MMP-2 from the extracellular milieu to the cell surface. The second step requires an active molecule of MT1-MMP, which itself activates MMP-2, leading to subsequent autocatalytic cleavage. Substrates of activated MMP-2 include several components of the articular cartilaginous matrix including: type IV collagen, aggrecan, link protein, decorin, fibronectin, and type X and XI collagens.

 

Elevated MMP-2 secretion is associated with poor prognosis in several types of human cancers. Mutations in the gene encoding MMP-2 gene are associated with Torg-Winchester syndrome, multicentric osteolysis, arthritis syndrome, and possibly keloids. MMP-2 deficient mice exhibit slightly delayed growth, reduced neovascularization, retarded tumor progression, an exaggerated asthma response to allergens, and impaired branching morphogenesis of the mammary glands.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MMP-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration of 1 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human MMP-2 protein ranging from 10 to 0.156 ng/ml are recommended for each ELISA plate when paired with M6303D01 (Cat. No. 679902). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated M6310C11 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M6303D01 (Cat. No. 679902) antibody as the capture antibody, and recombinant human MMP-2 (Cat. No. 763301) as a standard.

 

Note: For testing human MMP-2 in serum or plasma, BioLegend's LEGEND MAX™ Kits (Cat. No. 444607 & 444608) are specially developed and recommended.

RRID
AB_2728455 (BioLegend Cat. No. 532001)

Antigen Details

Structure
Monomer. Human MMP-2 contains 660 amino acids with a predicted molecular weight of 73.9 kD. Upon activation by p-aminophenylmercuric acetate (APMA), it has a predicted molecular weight of 62 kD.
Distribution

MMP-2 is expressed by fibroblasts, endothelial cells, alveolar epithelial cells, and macrophages.

Function
MMP-2 is a metalloproteinase that specifically cleaves type IV collagen, which is the major structural component of basement membranes. MMP2 is thought to contribute to the invasion and the metastasis of various human malignancies.
Interaction
Fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans, decorin, osteopontin, and IBP-3.
Ligand/Receptor
TIMPs, CCL7, and thrombospondin 1.
Cell Type
Fibroblasts, Endothelial cells, Epithelial cells, Macrophages
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Nagase H and Woessner JF. 1999. J. Biol. Chem. 274:21491.
  2. Martignetti JA, et al. 2001. Nat. Genet. 28:261.
  3. Itoh T, et al. 1997. J. Biol. Chem. 272:22389.
  4. Passlick B, et al. 2000. Clin. Cancer Res. 6:3944.
  5. Qian Q, et al. 2010. Cancer Invest. 28:661.
  6. Hoikkala S, et al. 2006. Cancer Lett. 236:125.
  7. Yamamura T, et al. 2002. Lung Cancer 35:249.
Gene ID
4313 View all products for this Gene ID
UniProt
View information about MMP-2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 04/09/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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