- 10C3 (See other available formats)
- Regulatory Status
- Other Names
- Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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Human macrophage migration inhibitory factor (MIF) is a 12.5 kD, 115 amino acid, non-glycosylated polypeptide expressed by multiple cell types, including activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. MIF plays many roles in biological processes such as catalytic activity, immunity, endocrine regulation, signal modulation, and inflammation.
MIF is expressed in malignant cells including lung, liver, breast, colon, and prostate tumors. Studies suggest that MIF might serve as a molecular link between chronic inflammation and cancer.
Recombinant MIF consists of a mixture of monomers, dimers, and trimers. The physiologically active forms are believed to be predominantly dimeric and trimeric forms.
- Antibody Type
- Host Species
- Recombinant human MIF with a His tag
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this protein is quality control tested by ELISA assay. A concentration of 0.125 µg/ml of the detection antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.
- Application Notes
ELISA: To measure human MIF, the purified 10C3 antibody (Cat. No. 525501) is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 10C3 antibody (Cat. No. 525503) as the detection antibody.
Additional reported applications (for the relevant formats) include: functional assay1 and neutralization1.The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
Note: For testing human MIF in serum, plasma or cell culture supernatant, BioLegend's LEGEND MAX™ ELISA Kits (Cat. No. 438407 & 438408) are specially developed and recommended.
(PubMed link indicates BioLegend citation)
- Zhou H, et al. 2009. PLoS ONE. 4:e6087. (FA, Neut)
AB_2563135 (BioLegend Cat. No. 525503)
AB_2563136 (BioLegend Cat. No. 525504)
- Predominantly a homotrimer
Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells
- Pro-inflammatory cytokine, regulates macrophage function, involved in cancer development, tautomerase activity
- Monocytes and macrophages, mesenchymal cells, B-cells
- CD74, CXCR4, CXCR2, CXCR7
- Cell Type
- Endothelial cells, Eosinophils, Epithelial cells, Macrophages, T cells
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- Antigen References
1. Zhou H, et al. 2009. PLoS ONE. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K & Iczkowski K. 2005. BMC Cancer. 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.
- Gene ID
- 4282 View all products for this Gene ID
- View information about MIF on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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