- 3.9 (See other available formats)
- Regulatory Status
- III NL707
- Other Names
- Integrin αX subunit, CR4, p150, ITGAX
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.Product Details
- Human, African Green, Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC)
- Ottonello L, et al. 1999. Blood 93:3505.
- Metelitsa LS, et al. 2002. Blood 99:4166.
- Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed
- Ihanus E, et al. 2007. Blood 109:802-810.
- Gurer C, et al. 2008. Blood 112:1231. PubMed
- Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)
- Product Citations
AB_493021 (BioLegend Cat. No. 301612)
- Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
- Myeloid, dendritic cells, NK cells, B cells and T cell subsets
- Adhesion, CTL killing
- CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
- Cell Type
- Dendritic cells, NK cells, B cells, T cells, Neutrophils, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810.
- Gene ID
- 3687 View all products for this Gene ID
- View information about CD11c on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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