Biotin anti-Allophycocyanin (APC) Antibody

Pricing & Availability
Clone
APC003 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
A_APC003_Biotin_111407
C57BL/6 thymocytes stained with RM4-5 APC only (top), or with RM4-5 APC plus biotinylated APC003 (bottom), then detected with Sav-FITC
  • A_APC003_Biotin_111407
    C57BL/6 thymocytes stained with RM4-5 APC only (top), or with RM4-5 APC plus biotinylated APC003 (bottom), then detected with Sav-FITC
  • B_APC003_Biotin_111407
Cat # Size Price Quantity Check Availability Save
408003 25 µg $45.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
408004 100 µg $100.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cy5.5 and APC/Cy7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
allophycocyanin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations comparable to those of the primary antibody of interest. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Coppieters K, et al. 2012. J Exp Med. 209:51:00. PubMed
  2. Illingworth J, et al. 2013. J Immunol. 190:1038. PubMed
  3. Matsushita H, et al. 2015. Cancer Immunol Res. 3:26. PubMed
  4. Marangoni F, et al. 2021. Cell. . PubMed
RRID
AB_345359 (BioLegend Cat. No. 408003)
AB_345360 (BioLegend Cat. No. 408004)

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account