APC anti-mouse Galectin-9 Antibody

Pricing & Availability
Clone
RG9-35 (See other available formats)
Regulatory Status
RUO
Other Names
Lgals9, Gal-9, Ecalectin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RG9-35_APC_Galectin-9 _Antibody_FC_100312
C57BL/6 mouse thymocytes were fixed, permeabilized and then stained with Galectin-9 (clone RG9-35) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • RG9-35_APC_Galectin-9 _Antibody_FC_100312
    C57BL/6 mouse thymocytes were fixed, permeabilized and then stained with Galectin-9 (clone RG9-35) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
See APC spectral data
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136109 25 µg $95.00
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136110 100 µg $245.00
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Description

Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc). Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It may be retained intracellularly or transported to the cell surface whereby cleavage generates a soluble form. Galectin-9 is involved in events such as cell aggregation, adhesion, chemotaxis, and apoptosis, and is important for the regulation of the immune response. Galectin-9 induces regulatory T cells, and suppresses Th1 and Th17 responses.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse galectin-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fukushima A, et al. 2008. Int. Arch. Allergy Immunol. 146:36. (FA)
  2. Hou H, et al. 2014. PLoS One. 9:110585. PubMed
RRID
AB_2561657 (BioLegend Cat. No. 136109)
AB_2561658 (BioLegend Cat. No. 136110)

Antigen Details

Structure
Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution
Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
Function
Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand/Receptor
Tim-3
Bioactivity
Chemotaxis, apoptosis, cell adhesion
Cell Type
Lymphocytes, Dendritic cells, Neutrophils, Eosinophils, Astrocytes, Endothelial cells, Fibroblasts, Epithelial cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Klibi J, et al. 2009. Blood 113:1957
2. Seki M, et al. 2008. Clin Immunol 127:78
3. Tsuboi Y, et al. 2007. Clin Immunol 124:221
4. Zhu C, et al. 2005. Nat Immunol 6:1245
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916

Gene ID
16859 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/10/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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