APC anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
10F.9G2 (See other available formats)
Regulatory Status
RUO
Other Names
B7-H1, PD-L1
Isotype
Rat IgG2b, κ
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Product Citations
publications
10Fdot9G2_APC_040610
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) APC (pink line) or rat IgG2b, κ APC (purple line).
  • 10Fdot9G2_APC_040610
    C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) APC (pink line) or rat IgG2b, κ APC (purple line).
See APC spectral data
Cat # Size Price Quantity Avail. Save
124311 25 µg $80
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124312 100 µg $245
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Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Maier H, et al. 2007. J. Immunol. 178:2714.
  2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
  3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
  4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
  5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
  6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
  7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
  8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
  9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
  10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
  12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations
  1. Poon E, et al. 2017. J Immunother Cancer. 10.1186/s40425-017-0268-8. PubMed
  2. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  3. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  4. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  5. Iwata TN, et al. 2018. Mol Cancer Ther. 17:1494. PubMed
  6. Wang C, et al. 2018. Sci Transl Med. 10. PubMed
  7. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  8. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  9. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  10. Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
  11. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  12. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  13. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  14. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  15. Pein M, et al. 2020. Nat Commun. 11:1494. PubMed
  16. Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed
  17. Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed
  18. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  19. Fukumoto T, et al. 2019. Cancer Res. 79:5482. PubMed
  20. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  21. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  22. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  23. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  24. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  25. Almishri W, et al. 2015. J Hepatol. 63: 943-951. PubMed
  26. Ngiow S, et al. 2016. Cancer Res . 76: 6266 - 6277. PubMed
  27. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  28. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  29. Chen L, et al. 2020. Cancer Immunol Res. 0.457638889. PubMed
  30. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  31. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  32. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  33. Lovisa S, et al. 2020. Sci Signal. 13:00. PubMed
RRID
AB_10612935 (BioLegend Cat. No. 124311)
AB_10612741 (BioLegend Cat. No. 124312)

Antigen Details

Structure
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/22/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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