Alexa Fluor® 647 anti-human IL-2 Antibody

Pricing & Availability
Clone
MQ1-17H12 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
Isotype
Rat IgG2a, κ
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Product Citations
publications
MQ1-17H12_ALX647_042606
PMA+ionomycin-stimulated (5 hours) human PBMCs surface stained with CD3 PE and intracellular stained with MQ1-17H12 Alexa Fluor® 647
  • MQ1-17H12_ALX647_042606
    PMA+ionomycin-stimulated (5 hours) human PBMCs surface stained with CD3 PE and intracellular stained with MQ1-17H12 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
500315 100 tests $225.00
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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cat (Feline)15 Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli - expressed recombinant human IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture2,3: The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.
Additional reported applications (for the relevant formats) include: immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.

Note: For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
  3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
  4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
  5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
  6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
  7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
  8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
  9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  12. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  13. Yeap SK, et al. 2013. BMC Complement Altern. Med. 13:145. (Neut)
  14. Wu Z, et al. 2015. J Virol. 89:6435. PubMed
  15. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed
Product Citations
  1. Bamidele AO, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:55. PubMed
  2. Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed
RRID
AB_493370 (BioLegend Cat. No. 500315)

Antigen Details

Structure
Cytokine; 15.4 kD (Mammalian)
Bioactivity
Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance
Cell Sources
T cells
Cell Targets
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Receptors
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Regulation
Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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