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Alexa Fluor® 594 anti-mouse CD100 (SEMA4D) Antibody

Pricing & Availability
Clone
BMA-12 (See other available formats)
Regulatory Status
RUO
Other Names
Neuroimmune semaphorin 4D, Sema4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Semaj, Semcl2, Coll-4, Semacl2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BMA_12_A594_CD100_Antibody_IHC_012122
C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-mouse CD100 (clone BMA-12) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • BMA_12_A594_CD100_Antibody_IHC_012122
    C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of anti-mouse CD100 (clone BMA-12) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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147609 25 µg $149
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147610 100 µg $358
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Description

CD100, also known as Semaphorin-4D (SEMA4D), is a 150 kD, type I transmembrane protein, with one sema domain and one C2-type Ig domain. It is found as a homodimer on the cell surface. CD100 is expressed in the brain, kidney, heart, the majority of hematopoietic cells, and some malignant cells. CD100 is a potent inducer of angiogenesis and inhibitor of bone formation, and plays a role in lymphocyte activation, antibody production, dendritic cell maturation, and axon guidance. Its ligands are CD72 and Plexin-B1. CD100 is cleaved from the membrane of activated T and B cells, producing a soluble form.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD100-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunohistochemistry, a concentration range of 5-10 μg/ml is suggested. For immunofluorescence microscopy, a concentration range of 5-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation from cell lysates2, capture antibody function in a sandwich ELISA assay2, and immunohistochemical staining of frozen brain sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Kumanogoh A, et al. 2000. Immunity. 13:621. (FC)
  2. Wang X, et al. 2001. Blood. 97:3498. (FC, IP, ELISA)
  3. Kumanogoh A, et al. 2002. J. Immunol. 169:1175. (FC)
  4. Moreau-Fauvarque C, et al. 2003 J. Neurosci. 23:9229. (IHC)
RRID
AB_2687303 (BioLegend Cat. No. 147609)
AB_2687304 (BioLegend Cat. No. 147610)

Antigen Details

Structure
Type I transmembrane protein, one sema domain, one C2-type immunoglobulin domain, homodimer, 150 kD.
Distribution

Highly expressed on most hematopoietic cells, is also found in the brain, kidney, and heart. Overexpressed by some malignant cell types. 

Function
Potent inducer of angiogenesis, inhibitor of bone formation, plays a role in lymphocyte activation, antibody production and dendritic cell maturation. In neurons, regulates dendrite branching and axon guidance.
Ligand/Receptor
CD72, Plexin-B1.
Cell Type
B cells, Dendritic cells, Neutrophils, Tregs
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 
3. Kishihara K, et al. 1993. Cell. 74:143. 
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
20354 View all products for this Gene ID
UniProt
View information about CD100 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05/31/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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