- TER-119 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Day-14 fetal liver cells from a C57BL/6 mouse
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
IHC-F, IHC-P, IP, WB, CMCD - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).
(PubMed link indicates BioLegend citation)
- Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
- Vannucchi AM, et al. 2000. Blood 95:2559.
- Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
- Grisendi S, et al. 2005. Nature 437:147. (FC)
- Bourdeau A, et al. 2007. Blood 109:4220.
- Chappaz S, et al. 2007. Blood 110:3862. (FC)
- Heuser M, et al. 2007. Blood 110:1639. (FC)
- Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
AB_2832399 (BioLegend Cat. No. 116253)
AB_2832400 (BioLegend Cat. No. 116254)
AB_2832401 (BioLegend Cat. No. 116255)
AB_2832402 (BioLegend Cat. No. 116256)
AB_2832403 (BioLegend Cat. No. 116257)
AB_2832404 (BioLegend Cat. No. 116258)
- Associated with glycophorin A, 52 kD
Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
- Cell Type
- Biology Area
- Antigen References
1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.
- Gene ID
- 104231 View all products for this Gene ID
- View information about TER-119 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse TER-119/Erythroid Cells
Biotin anti-mouse TER-119/Erythroid Cells
FITC anti-mouse TER-119/Erythroid Cells
PE anti-mouse TER-119/Erythroid Cells
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells
Purified anti-mouse TER-119/Erythroid Cells
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells
PerCP anti-mouse TER-119/Erythroid Cells
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells
Pacific Blue™ anti-mouse TER-119/Erythroid Cells
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody