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Ultra-LEAF™ Purified anti-mouse CD96 (TACTILE) Antibody

Pricing & Availability
Clone
W20256A (See other available formats)
Regulatory Status
RUO
Other Names
T-cell surface protein tactile, TACTILE, Cell surface antigen CD96, T cell-activated increased late expression protein
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20256A_ULEAF_CD96_Antibody_061023
Recombinant mouse CD96 (TACTILE) (Cat. No. 788804) (black circles) binds to immobilized recombinant mouse CD155-Fc Chimera (Cat. No. 786606). Ultra-LEAF™ purified anti-mouse CD96 (TACTILE) (clone W20256A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2b, κ isotype control (Cat. No. 400666) (gray triangles) does not have the effect. This antibody blocks the binding of 0.5 µg/mL mouse CD96 (TACTILE) to 2 µg/mL immobilized recombinant mouse CD155-Fc Chimera. ND50: 0.1-1 µg/mL.
  • W20256A_ULEAF_CD96_Antibody_061023
    Recombinant mouse CD96 (TACTILE) (Cat. No. 788804) (black circles) binds to immobilized recombinant mouse CD155-Fc Chimera (Cat. No. 786606). Ultra-LEAF™ purified anti-mouse CD96 (TACTILE) (clone W20256A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2b, κ isotype control (Cat. No. 400666) (gray triangles) does not have the effect. This antibody blocks the binding of 0.5 µg/mL mouse CD96 (TACTILE) to 2 µg/mL immobilized recombinant mouse CD155-Fc Chimera. ND50: 0.1-1 µg/mL.
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618753 100 µg $358
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618754 1 mg $1205
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Description

CD96, also known as TACTILE (T cell-activated increased late expression), is a type I transmembrane glycoprotein, belonging to the Ig superfamily. It is expressed on NK cells and T cells (upregulated after activation), but not on normal B cells and myeloid cells. CD96 is also found on some acute myeloid leukemia (AML) cells, and T cell acute lymphoblastic leukemia (T-ALL), as a leukemia stem cell-specific marker. A soluble form of CD96 has been found in serum. Human CD96, but not mouse CD96, is expressed as two isoforms due to alternative splicing. The main ligand of CD96 is CD155 (PVR), along with CD226 (DNAM-1) and TIGIT, and the binding is species-specific. Mouse CD96 has been reported to interact with nectin-1 (CD111), but human CD96 does not. CD96 may play an inhibitory role in immune responses. In CD96 deficient mice, NK cells produce more IFN-γ upon LPS treatment. In addition, CD96 negatively regulates NK cell function in mice, and blockade of CD96 with antibodies promotes anti-tumor responses and anti-metastatic activity in combination with anti-CTLA-4 or anti-PD-1. CD96 is considered to mediate activated T cell and NK cell adhesion to CD155-expressing target cells. CD96 also counterbalances CD226, which activates NK cell-mediated cytotoxicity, via competing CD155 binding.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD96 (TACTILE)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.5 µg/mL recombinant mouse CD96 (TACTILE) (Cat. No. 788804) to immobilized 2 µg/mL recombinant mouse CD155-Fc Chimera (Cat. No. 786606).  ND50 = 0.1 - 1 µg/mL.

RRID
AB_3068077 (BioLegend Cat. No. 618753)
AB_3068077 (BioLegend Cat. No. 618754)

Antigen Details

Structure
Ig superfamily
Distribution

Positive on most peripheral T cells, NK cells and upregulated when activated

Function
CD96 is involved in NK cell adhesion and cytotoxicity via CD155
Ligand/Receptor
CD155, nectin 1
Cell Type
NK cells, NKT cells, T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
Soluble Receptors
Antigen References
  1. Wang PL, et al. 1992. J Immunol. 148:2600-8. 
  2. Meyer D, et al. 2009. J Biol Chem. 284:2235-44. 
  3. Hosen N, et al. 2007. Proc Natl Acad Sci USA. 104:11008-13.
  4. Wen D, et al. 2015. Mol Clin Oncol. 3:833-838.
  5. Blake SJ, et al. 2016. Clin Cancer Res. 22:5183-5188.
  6. Chan CJ, et al. 2014. Nat Immunol. 15:431-8. 
  7. Sanchez-Correa B, et al. 2019. Cancer. 11:877. 
  8. Xu Z, Jin B. 2010. Cell Mol Immunol. 7:11-9.
  9. Georgiev H, et al. 2018. Front Immunol. 9:1072. 
  10. Fuchs A, et al. 2004. J Immunol. 172:3994-8.
  11. Seth S, et al. 2007. Biochem Biophys Res Commun. 364:959-65.
Gene ID
84544 View all products for this Gene ID
UniProt
View information about CD96 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/09/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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