Ultra-LEAF™ Purified anti-human CRP (C-reactive protein) Antibody

Pricing & Availability
Clone
A16065C (See other available formats)
Other Names
C-reactive protein (CRP), Pentraxin 1 (PTX1)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16065C_ULEAF_CRP_Antibody_033017
Immobilized recombinant human CRP at 2 µg/mL binds recombinant human APCS (grey circles). Anti-human CRP antibody (clone A16065C, purple squares) inhibits the binding of recombinant human APCS (at 1 µg/mL) in a dose dependent manner as detected by functional ELISA. The ND50 = 0.4 - 2.0 µg/mL.
  • A16065C_ULEAF_CRP_Antibody_033017
    Immobilized recombinant human CRP at 2 µg/mL binds recombinant human APCS (grey circles). Anti-human CRP antibody (clone A16065C, purple squares) inhibits the binding of recombinant human APCS (at 1 µg/mL) in a dose dependent manner as detected by functional ELISA. The ND50 = 0.4 - 2.0 µg/mL.
Cat # Size Price Quantity Avail. Save
696003 100 µg $235
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696004 1 mg $650
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Description

C-reactive protein (also known as CRP and Pentraxin 1 (PTX1)), is a member of pentraxin protein family. Similar to other pentraxins, CRP is a pattern recognition molecule that is able to recognize pathogens and promote their clearance. CRP tends to form a non-covalently linked pentamer with each subunit made up of two antiparallel β-sheets and a single short α-helix. It is characterized by the presence of a cleft that extends from the center of the subunit to its edge at the central pore of the pentamer structure. It has been demonstrated that CRP binds to the phosphocholine moieties expressed on the surface of dead or dying cells and some bacteria. This leads to the activation of the complement system and promotion of phagocytosis by macrophages to clear necrotic and apoptotic cells. CRP is secreted by hepatocytes and is a biomarker for inflammation. IL-6 has been shown to be an effective inducer of this protein. Besides binding to apoptotic cells, CRP also binds to a variety of molecules including C1q, bacterial polysaccharide, CD64, CD32, APCS, HDL, and fibronectin. In addition to participating in immune responses, CRP is also a prominent partaker in endothelial dysfunction and atherosclerosis. It was shown that CRP may exhibit direct proantherogenic effects by upregulating angiotensin type I receptor in smooth muscle cells and promoting their migration, proliferation, neointimal formation, and reactive oxygen species production. Mature human CRP shares 71% amino acid sequence homology with its mouse counterpart.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CRP/PTX1
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of recombinant human APCS (1 µg/mL) to immobilized recombinant human CRP (2 µg/mL). ND50 range:  0.4 - 2.0 mg/mL.  It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2686982 (BioLegend Cat. No. 696003)
AB_2686983 (BioLegend Cat. No. 696004)

Antigen Details

Structure
Monomer and homopentamer
Distribution

Hepatocytes

Function
Induces expression of E-selectin and VCAM-1 expression in epithelial cells. CRP binds to target, initiates, and regulates complement activation. Induced by IL-1 and IL-6.
Interaction
Phosphocholine on the surface of apoptotic cells
Ligand/Receptor
Interacts with C1q, bacterial polysaccharide, CD64, CD32, APCS (PTX2), HDL, leptin, and fibronectin.
Biology Area
Cell Biology
Antigen References

1. Ansar W, Ghosh S. 2013. Immunol. Res. 56:131.
2. Agrawal A, et al. 2009. Adv. Exp. Med. Biol. 653:98.
3. Garlanda C, et al. 2005. Annu. Rev. Immunol. 23:337.
4. Verma S, et al. 2004. Circulation 109:1914.
5. Wadham C, et al. 2004. Circulation 109:2116.
6. Li XA, et al. 1998. Biochem. Biophys. Res. Commun. 244:249.
7. Lu J, et al. 2012. Immunol. Rev. 250:230.
8. Chen K, et al. 2006. Nat. Med. 12:425.

Gene ID
1401 View all products for this Gene ID
UniProt
View information about CRP on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 0    Revision Date: 04/10/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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