Ultra-LEAF™ Purified anti-human CD314 (NKG2D) Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation^1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation^2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)

1. Wu J, et al. 1999. Science 285:730.
2. Wu J, et al. 2000. J. Exp. Med. 192:1059.
3. Groh V, et al. 2001. Nature Immunol. 2:255.
4. Wu J, et al. 2002. J. Immunol. 169:1236.
5. Roberts A, et al. 2001. J. Immunol. 167:5527.
6. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
7. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
8. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
9. Watanbe M, et al. 2014. Int Immunol. PubMed

Product Citations

1. Breman E, et al. 2019. Front Immunol. 9:2940. PubMed
2. Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed
3. Dudek M, et al. 2021. Nature. 592:444. PubMed
4. Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
5. Glasner A, et al. 2017. Sci Rep. 7:40944. PubMed

RRID

AB_2810480 (BioLegend Cat. No. 320813)
AB_2561488 (BioLegend Cat. No. 320814)

Antigen Details

Structure

C-type lectin

Distribution

NK cells, γ/δ T cells, CD8⁺ T cells

Function

Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells

Ligand/Receptor

MICA, MICB, UL16-binding proteins (ULBPs)

Cell Type

NK cells, T cells

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Jamieson AM, et al. 2002. Immunity 17:19.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
7. Maasho K, et al. 2005. J. Immunol. 174:4480.
8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.

Gene ID

22914 View all products for this Gene ID

UniProt

View information about CD314 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis