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Ultra-LEAF™ Purified anti-human CCL21 (6CKine) Antibody

Pricing & Availability
Clone
A15161B (See other available formats)
Regulatory Status
RUO
Other Names
6CKine, Secondary lymphoid tissue chemokine, SLC, Exudos 2, Small inducible cytokine subfamily A, member SCYA21
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A15161B_ULEAF_CCL21_Antibody_030722
Recombinant human CCL21 (6CKine) (Cat. No. 582202) (black circles) induces the chemotaxis of mouse Baf3-hCCR7 transfectants. Ultra-LEAF™ purified anti-human CCL21 (6CKine) (clone A15161B) (purple squares) neutralizes the chemotaxis induced by recombinant human CCL21 (6CKine) at 6 ng/mL on mouse Baf3-hCCR7 cells in a dose-dependent manner, whereas the GoInVivo™ purified mouse IgG1, κ isotype control (Cat. No. 400192) (gray triangles) does not have the effect. ND50 range: 0.06 - 0.6 µg/mL
  • A15161B_ULEAF_CCL21_Antibody_030722
    Recombinant human CCL21 (6CKine) (Cat. No. 582202) (black circles) induces the chemotaxis of mouse Baf3-hCCR7 transfectants. Ultra-LEAF™ purified anti-human CCL21 (6CKine) (clone A15161B) (purple squares) neutralizes the chemotaxis induced by recombinant human CCL21 (6CKine) at 6 ng/mL on mouse Baf3-hCCR7 cells in a dose-dependent manner, whereas the GoInVivo™ purified mouse IgG1, κ isotype control (Cat. No. 400192) (gray triangles) does not have the effect. ND50 range: 0.06 - 0.6 µg/mL
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604353 100 µg $413
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604354 1 mg $1205
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Description

CCL21 is a CC chemokine highly expressed in lymph nodes. CCL21 is only 32% identical to CCL19 at the amino acid level; nevertheless, these chemokines bind to the same receptor CCR7.  CCL21 has a unique extension of about 30 amino acids with two extra cysteine residues in its carboxyl terminus. CCL21 is expressed in synovial tissue in rhematoid arthritis (RA), and it has been suggested that CCL21, but not CCL19, induces human microvascular endothelial cell (HMVEC) migration and/or tube formation. In addition, it has been reported that sustained high concentrations of either soluble or immobilized CCL19 and CCL21 inhibit T cell proliferation and IL-2 secretion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL21 (6CKine)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant human CCL21 (6CKine) (Cat. No. 582202) at 6 ng/mL on mouse Baf3- hCCR7 transfectants. ND50 range: 0.06 - 0.6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922638 (BioLegend Cat. No. 604353)
AB_2922638 (BioLegend Cat. No. 604354)

Antigen Details

Structure
Chemokine
Distribution

CCL21 is highly expressed in HEV of lymph nodes and Peyer's patches. Also, it is expressed in the T cell zones of lymph nodes, in Peyer's patches by reticular cells, and in spleen by stromal cells. CCL21 is expressed by endothelial cells in lymphatic venules.

Function
CCL21 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL21 and CCL19 chemoattract antigen-engaged B cells to the boundary of B and T zones to interact with T-helper cells. CCL19 and CCL21 inhibit apoptosis in DCs.
Interaction
Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA)
Ligand/Receptor
CCR7 (CD197)
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Hedrick JA and Zlotnik A. 1997. J. Immunol. 159:1589.
  2. Reif K, et al. 2002. Nature 416:94.
  3. Ziegler E, et al. 2007. J. Immunol. 179:6485.
  4. Pickens SR, et al. 2012. Arthritis Rheum. 10.1002.
Gene ID
6366 View all products for this Gene ID
UniProt
View information about CCL21 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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