TotalSeq™-Bn0037 anti-human/mouse/rat PCNA Antibody

Pricing & Availability
Clone
PC10 (See other available formats)
Regulatory Status
RUO
Other Names
Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
Isotype
Mouse IgG2a, κ
Barcode Sequence
CATATCTCTCACTCG
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Product Citations
publications
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307915 10 µg $369
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Description

The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant rat PCNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-Bn oligomer under optimal conditions.
Concentration
0.5 mg/mL
Application

SB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining in formalin-fixed, paraffin-embedded (FFPE) lymphoid tissue, and the oligomer sequence is confirmed by sequencing. TotalSeq™-Bn antibodies are compatible with the 10x Visium CytAssist Gene and Protein Expression Assay.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution at 14,000xg at 2 − 8°C for 10 minutes before use. Carefully pipette out the liquid avoiding the bottom of the tube when handling. To determine and optimize dilutions for the addition of Totalseq™-Bn antibodies into pre-designed antibody panels, refer to 10x Genomics Custom Add-on Antibody Optimization guide.


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.

Additional Product Notes

TotalSeq™-Bn reagents are designed to profile protein levels following an optimized protocol in spatial transcriptomics. Compatible spatial biology devices (e.g. Imaging System, 10x Genomics Visium Spatial CytAssist Gene and Protein Expression instruments and reagents) and sequencer (e.g. Illumina analyzers) are required. TotalSeq™-B reagents are not compatible with the 10x Genomics Visium system. The complete barcode sequence may be provided upon request. Please contact technical support for more information, or visit TotalSeq™-Bn Reagents for 10x Genomics Visium CytAssist Gene and Protein Assay

Application References

(PubMed link indicates BioLegend citation)
  1. Ogata K, et al. 1985. J. Immunol. 135:2623.
  2. Garcia R, et al. 1989. Am. J. Pathol. 134:733.
  3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
  4. Waseem N, et al. 1990. J. Cell Sci. 96:121.
  5. Yu C, et al. 1991. Histopathology 19:29.
  6. Wilkins B, et al. 1992. J. Pathol. 166:45.
  7. Yang W, et al. 1996. Human Pathol. 27:70.
  8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
  10. Fulvio MD, et al. 2006. Oncogene 25:3932.
  11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
  12. Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
  13. Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
  14. Wallace HA, et al. 2014. Development. 141:1332. PubMed
  15. Mizokami A, et al. 2014. Bone. 69:68. PubMed
RRID
AB_3083171 (BioLegend Cat. No. 307915)

Antigen Details

Structure
DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution

Nuclear, all proliferating cells

Function
RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Interaction
PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Ligand/Receptor
Ubiquitination, Sumoylation
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Nuclear Markers
Antigen References

1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.

Gene ID
5111 View all products for this Gene ID
UniProt
View information about PCNA on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 1    Revision Date: 08/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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