Recombinant Human Osteopontin (carrier-free)

Pricing & Availability
Regulatory Status
Other Names
Ave. Rating
Submit a Review
Product Citations
Human osteopontin inhibits the production of IFN-γ in human activated T cells in a dose-dependent manner. The ED50 for this effect is 0.2 – 2.0 ng/mL.
  • Osteopontin_Human_RECOM_BA_1111022
    Human osteopontin inhibits the production of IFN-γ in human activated T cells in a dose-dependent manner. The ED50 for this effect is 0.2 – 2.0 ng/mL.
Cat # Size Price Quantity Check Availability Save
557102 10 µg $89
Check Availability

Need larger quantities of this item?
Request Bulk Quote
557104 25 µg $147
Check Availability

Need larger quantities of this item?
Request Bulk Quote
557106 100 µg $347
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Osteopontin, also known as secreted phosphoprotein 1 (SPP1) and early T lymphocytes activation 1 (ETA-1), is an arginine-glycine-aspartic acid (RGD) that contains multi-functional glycoproteins who are able to interact with integrins and CD44 as receptors. This protein has been shown to play roles in cell migration and survival, bone remodeling, regulation of immune cell functions, apoptosis, control of tumor cell phenotypes, and autoimmune diseases. Osteopontin is involved in recruiting and stimulating macrophages and lymphocytes in response to microbial infections. The Osteopontin receptor-ligand interaction mediates chemotaxis or attachment that depends on whether the protein is in soluble or immobilized form. Osteopontin has also been shown to play a role in the progression of cancer, which is supported by the finding that patients who suffer from disseminated carcinoma also have increased levels of Osteopontin in their plasma. In addition, Osteopontin is a component of the human atherosclerotic plaque and may be a mediator of arterial neointima formation. It is synthesized by macrophage, smooth muscle, and endothelial cells in primary and recurrent human coronary atherosclerotic plaques, which may contribute to the cellular accumulations and dystrophic calcification in the atherosclerotic plaques. Osteopontin is also a substrate for MMP-3, MMP-7, MMP-9, MMP-12, and thrombin within the SVVYGLR motif.

Product Details
Technical data sheet

Product Details

Human Osteopontin, amino acids (Ile17-Asn300) (Accession# NP_000573.1), was expressed in 293E cells with a C-terminal His tag.
Molecular Mass
The 290 amino acid recombinant protein has a predicted molecular mass of approximately 33 kD. The DTT-reduced and non-reduced protein migrates approximately 55 kD by SDS-PAGE. The predicted N-terminal amino acid is Ile.
>95%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 1.0 EU per µg of protein as determined by the LAL method.
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Human osteopontin inhibits the production of IFN-γ in human activated T cells in a dose-dependent manner. The ED50 for this effect is 0.2 – 2.0 ng/mL.


Application Notes

Bioassay Protocol1

  1. Increasing concentrations of hOsteopontin, along wtih 500 ng/ml of anti-CD3 antibody (OKT3), were used to coat a 96-well plate overnight.
  2. Excess hOsteopontin and OKT3 were washed away with PBS before incubating purified human T cells at 2 X 105 cells per well.
  3. The supernatant cell culture was collected after 24 - 48 hours of incubation and the concentration of IFN-γ was detected using BioLegend's Human IFN-γ ELISA MAX™ Deluxe (Cat No: 430104).

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at

Application References

(PubMed link indicates BioLegend citation)
  1. O'Regan AW, et al. 2000. J. Leuko. Biol. 68:495.

Antigen Details


Osteoblasts, osteoclasts, endothelial cells, macrophages, and smooth muscle cells.

Plays a role in cell migration, survival, inhibition of calcification, and regulation of immune cell function. Hypocalcemia and hypophosphatemia leads to an increase in Osteopontin transcription, translation, and secretion.
T cells, neutrophils, macrophages, osteoclasts, and mast cells.
CD44, integrins (αVβ3).
Human OPN1 inhibits IFN-γ secretion by anti-CD3 activated human T cells.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Signal Transduction, Stem Cells
Molecular Family
Antigen References

1. Wang KX and Denhardt DT. 2008. Cytokine Growth Factor Rev. 333.
2. Agnihotri R, et al. 2001. J. Biol. Chem. 276:28261.
3. O'Regan AW, et al. 2000. J. Leuko. Biol. 68:495.
4. Yokosaki Y, et al. 2005. Matrix Biol. 24:418.
5. Rangaswami H, et al. 2006. Trends Cell Biol. 16:79.

Gene ID
6696 View all products for this Gene ID
View information about Osteopontin on

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 2    Revision Date: 11/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Remember me
Forgot your password? Reset Password
Request an Account