Recombinant Human IL-8 (Animal-Free)

Pricing & Availability
Other Names
Small inducible cytokine subfamily B, member 8 (SCYB8), Monocyte derived neutrophils chemotactic factor (MDNCF), Neutrophil-activating peptide 1 (NAP1), Granulocyte chemotactic protein 1 (GCP1), Chemokine, CXC motif, ligand 8, CXCL8
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715404 25 µg $215
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Description

IL-8 (CXCL8) was originally recognized as a neutrophil-activating protein on the basis of two in vitro effects, chemotaxis and the release of granule enzymes. IL-8 is generated as a precursor of 99 amino acids. Once the mature cytokine is secreted, the amino-terminal is processed to yield several biologically active variants. The predominant variant consists of 72 amino acids. IL-8 is one of the ELR (Glu-Leu-Arg) motif-positive CXC-chemokines and shares similarities with angiogenic cytokines.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human IL-8, amino acids Ser28-Ser99 (Accession# NM_000584) was expressed in E. coli.
Molecular Mass
The 72 amino acid recombinant protein has a predicted molecular mass of 8.4 kD. The predicted N-terminal amino acid is Ser.
Purity
>98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation
Lyophilized, carrier-free.
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract human peripheral blood neutrophils in a dose dependent manner. The chemotactic activity was detected from 10 to 100 ng/ml.
Application

Bioassay

Antigen Details

Structure
Chemokine
Distribution
IL-8 is expressed by neutrophils, mococytes, macrophages, mast cells, endothelial cells, fibroblasts, keratinocytes, synovial cells, chondrocytes, epithelial cells, megakariocytes, melanocytes, hepatocytes, mesangial, and tumor cells. Most primary and metastatic solid tumors, such as breast, uterine, prostate, colon, and pancreatic carcinomas, melanoma, and glioblastoma, constitutively express IL-8.
Function
CXCL8 has diverse functions in immune surveillance, inflammation, and angiogenesis. CXCL8 directly modulates endothelial cell proliferation and migration, thus promoting angiogenesis. IL-8 modulates myelopoiesis by suppressing the myeloid progenitor proliferation. IL-8 also modulates the homing of hematopoitic progenitors by affecting the secretion of MMPs and TIMPs from bone marrow and peripheral blood CD34+ cells. Monocytes and macrophages generate IL-8 upon stimulation with IL-1α, IL-1β, TNF-α, IL-3, GMCSF, endotoxin, lectins, phorbol esters, immune complexes, and phagocytosis. Opsonized particles are also a prime stimulus for IL-8 production by neutrophils.
Interaction
Neutrophils, basophils, highly cytotoxic CD8 T cells, megakariocytes, endothelial cells.
Ligand/Receptor
CXCR1 (CDw128a) and CXCR2.
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hess C, et al. 2004. Blood 104:3463.
2. Emadi S, et al. 2005. Blood 105:464.
3. Matsuo Y, et al. 2009. Int. J. Cancer 4:853.
4. Drewniak A, et al. 2010. Blood 115:4588.

Gene ID
3576 View all products for this Gene ID
UniProt
View information about IL-8 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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