Recombinant Human GAS6 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Growth arrest specific 6, AXSF (AXL stimulatory factor), AXLLG (AXL receptor tyrosine kinase ligand)
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Human_GAS6_RECOM_1_022924
Immobilized recombinant human GAS6 binds to biotinylated human Axl in a dose-dependent manner. The ED50 for this effect is 1.2 -7.2 ng/mL.
  • Human_GAS6_RECOM_1_022924
    Immobilized recombinant human GAS6 binds to biotinylated human Axl in a dose-dependent manner. The ED50 for this effect is 1.2 -7.2 ng/mL.
  • Human_GAS6_RECOM_2_022924
    Stability testing for Recombinant human GAS6. Recombinant human GAS6 was aliquoted in PBS containing 25% glycerol, pH 7.2 at 0.2 mg/ml and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to biotinylated human Axl in a dose-dependent manner. The ED50 for this effect is 1.2 - 7.2 ng/mL.
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796806 100 µg $516
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Description

First described in 1993, Growth arrest-specific gene 6 (Gas6) has 44% identity to human protein S, a vitamin K-dependent negative co-regulator in the blood coagulation pathway. Similar to protein S, Gas6 is composed of defined structural motifs comprised of a gamma-carboxylated amino terminus (Gla domain), four tandem repeats of epidermal growth factor (EGF)-like domains and a large carboxy-terminal domain with similarity to the sex hormone-binding globulin (SHBG). Gas6 has been identified as a ligand for the Axl/Tyro3 family of receptor tyrosine kinases (RTK), including Axl, Tyro3 (also known as Sky) and Mer. Overexpression of receptors for Gas6 belonging to the Axl/Tyro3 family has been reported in human mammary tumors, leukemia and lung cancers. Gas6 has been shown to amplify platelet response which plays a significant role in pathological thrombosis. Human platelets express all three RTKs (Axl, Tyro3 and Mer) and human blood has high Gas6 expression. Role of GAS6/Axl system as an early marker of disease prognosis in COVID-19 patients has been recently established.

Product Details
Technical data sheet

Product Details

Source
Human GAS6, amino acid (Ala49-Ala678) (Accession: NM_000820.2), with a C-terminal 8His tag, was expressed in CHO cells.
Molecular Mass
The 643 amino acid recombinant protein has a predicted molecular mass of approximately 71.25 kD. The DTT-reduced and non-reduced glycosylated protein migrate at approximately 70 kD and 60 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in 25% Glycerol, PBS pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2ᵒC and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Immobilized recombinant human GAS6 binds to biotinylated recombinant human Axl in dose-dependent manner. The ED50 for this effect is 1.2 – 7.2 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

Bone marrow stromal cells, Leydig cells, osteoblasts, tumor-associated macrophages, alveolar cells

Function
Cell proliferation, angiogenesis, erythropoiesis, regulation of inflammation, platelet stabilization, maintenance of vascular smooth muscle homeostasis, spermatogenesis, maintenance of the retina, regulation of osteoclast function, and cancer progression. GAS6 secretion is stimulated in vascular cells by collagen, erythropoietin, thrombin and inflammatory cytokines
Interaction
Phosphatidylserine in apoptotic cells, macrophages, monocytes, dendritic cells, NK cells, platelets, endothelial cells, osteoblasts, and fibroblasts
Ligand/Receptor
AXL, MERTK, and TYRO3
Bioactivity
Immobilized recombinant human GAS6 binds to biotinylated recombinant human Axl in dose-dependent manner. The ED50 for this effect is 1.2 – 7.2 ng/mL.
Cell Type
Dendritic cells, Endothelial cells, Fibroblasts, Macrophages, Mesenchymal Stem Cells, Monocytes, NK cells, Osteoblasts, Platelets, Th17
Biology Area
Angiogenesis, Cardiovascular Biology, Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Stem Cells
Molecular Family
Growth Factors
Antigen References
  1. Manfioletti G, et al. 1993 Mol Cell Biol 13: 4976-4985
  2. O Mc Cormack, et al. 2008. British Journal of Cancer. 98:1141-6.
  3. Varnum BC, et al. 1995. Nature. 373:623-6.
  4. Crosier PS, et al. 1995. Leuk Lymphoma. 18:443-9.
  5. Berclaz G, et al. 2001. Ann Oncol. 12:819-24.
  6. Shieh YS, et al. 2005. Neoplasia. 7:1058-64.
  7. Gould WR, et al. 2005. J Thromb Haemost. 3:733-41.
  8. Balogh I, et al. 2005. Arterioscler Thromb Vasc Biol. 25:1280-6.
  9. Morales A, et al. 2021. Biomedicines. 9:335.
  10. Tae Hoon Kim, et al. 2023. Life Sci. 10:122230.
Gene ID
2621 View all products for this Gene ID
UniProt
View information about GAS6 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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