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Purified anti-TRAF1 Antibody

Pricing & Availability
Clone
W15137A (See other available formats)
Regulatory Status
RUO
Other Names
TNF receptor associated factor 1, EBI6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W15137A_PURE_TRAF1_Antibody_WB_082916
Total cell lysates (15 µg protein) from Raji (lane 1), Daudi (lane 2) and 293E (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-TRAF1 antibody, clone W15137A (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for anti-TRAF1 antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: Molecular weight ladder.
  • W15137A_PURE_TRAF1_Antibody_WB_082916
    Total cell lysates (15 µg protein) from Raji (lane 1), Daudi (lane 2) and 293E (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-TRAF1 antibody, clone W15137A (upper panel). Proteins were visualized by chemiluminescence detection (Cat. No. 426303) using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for anti-TRAF1 antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: Molecular weight ladder.
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689802 100 µg $253
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Description

TNF Receptor Associated Factor 1 (TRAF1) is a member of the TRAF protein family, which mediates the downstream signaling of the TNFR superfamily, such as CD40, CD30, TNFR1 and TNFR2. Upon stimulation of TNF-α, TRAF1 forms a heterodimer with TRAF2 and subsequently activates MAPK/JNK and NFkB pathways. The TRAF1/TRAF2 complex also interacts with apoptosis inhibitor protein IAPs and mediates the anti-apoptotic signaling from TNF receptor. This heterodimer is part of the antiapoptotic E3 ubiquitin-protein ligase complex which promotes ubiquitination of target proteins, such as MAP3K14. TRAF proteins function as adaptor proteins for many cell surface receptors and participate in cell survival, proliferation and differentiation. Defects in TRAF1 have been shown to associate with hodgkin lymphoma and rheumatoid arthritis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human TRAF1 recombinant protein (1-182 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629790 (BioLegend Cat. No. 689802)

Antigen Details

Structure
416 amino acids with a predicted molecular weight of 46 kD. Contains a C-terminal MATH/TRAF domain responsible for binding to receptor cytoplasmic domains.
Distribution

Cytoplasm.

Function
TRAF1 is an adaptor protein, mediating downstream signaling of the TNFR superfamily. It plays a role in the regulation of cell survival, proliferation, and differentiation.
Interaction
TRAF1 forms a homotrimer and a heterodimer with TRAF2. Interacts with TRAIP, TANK, RIPK2, BIRC2, IRC3, NFATC2IP, TRAFD1, HIVEP3, MAP3K14, and TNFR family members.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Kim CM, et al. 2016. Sci. Rep. 6:25526.
2. Greenfeld H, et al. 2015. PLoS Pathog. 11:e1004890.
3. Lu YY, et al. 2013. Nat. Commun. 4:2852.
4. McPherson AJ, et al. 2012. J. Biol. Chem. 287:23010.
5. Wang C, et al. 2012. J. Exp. Med. 209:77.
6. Lee SY, et al. 2007. Adv. Exp. Med. Biol. 597:25.

Gene ID
7185 View all products for this Gene ID
UniProt
View information about TRAF1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/31/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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