Purified anti-TDP43 Phospho (Ser409/410) Antibody (Previously Covance catalog# SIG-39852)

Pricing & Availability
Clone
1D3/TDP-43 (See other available formats)
Other Names
TAR DNA-binding protein-43, TDP 43, TARDBP, ALS10
Previously
Covance Catalog# SIG-39852
Isotype
Rat IgG2a
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
829901 100 µL $309
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Description

TDP43 (aka TARDBP) was originally identified as a protein which binds to the transactivation response (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. TDP43 contains two copies of a ~90 amino acid RNA recognition motif (RRM) found in many proteins which bind single stranded RNA. TDP-43 localizes to the nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.  Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. Hyperphosphorylated, ubiquitinated, and cleaved in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This antibody was raised against a phosphopeptide corresponding to: i) the C-terminal domain of human TDP43; and ii) inclusive of phosphorylated serine residues 409 and 410.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC, IF, ELISA- Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000
 

Application Notes

This antibody is effective in immunoblotting (WB)

Positive Control: Rat Brain nuclear lysate
Expected MW: ~43 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Neumann, M., et al., Phosphorylation of S409/410 of TDP-43 is a consistent feature in all sporadic and familial forms of TDP-43 proteinopathies. Acta Neuropathol 117(2):137-149; 2009.
  2. Sarroukh R, et al. 2011. Cell Mol Life Sci. 68:1429. (WB, IHC, ELISA, IF)
  3. Ramakrishnan M, et al. 2009. Biochemistry 48:10405. (WB)
  4. Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:10323. (WB, IHC)
  5. Diomede L, et al. 2014. Neurobiol Dis. 62:521. (IHC)
Product Citations
  1. Wiesner D, et al. 2017. Exp Neurol. 10.1016/j.expneurol.2017.09.011. PubMed
  2. Gasset‐Rosa F et al. 2019. Neuron. 102(2):339-357 . PubMed
Publication Library
RRID
AB_2564934 (BioLegend Cat. No. 829901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins, TDP43
Gene ID
23435 View all products for this Gene ID 298648 View all products for this Gene ID
UniProt
View information about TDP43 Phospho (Ser409/410) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/17/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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