Purified anti-Tau, 15-25 Antibody

Pricing & Availability
Clone
TAU-13 (See other available formats)
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1 gamma2 subunit-interacting factor 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Tau‐13_PURE_Tau_032718
Western blot of anti-Tau, 15-25 antibody (clone Tau-13). Lane 1: Molecular weight marker; Lane 2: 20 µg of Alzheimer's disease human brain membrane lysate; Lane 3: 20 µg of normal human membrane lysate. The blot was incubated with 0.1 µg/mL of primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • Tau‐13_PURE_Tau_032718
    Western blot of anti-Tau, 15-25 antibody (clone Tau-13). Lane 1: Molecular weight marker; Lane 2: 20 µg of Alzheimer's disease human brain membrane lysate; Lane 3: 20 µg of normal human membrane lysate. The blot was incubated with 0.1 µg/mL of primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
835203 25 µg $95
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835204 100 µg $235
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Preliminary assignment of its epitope indicates that it maps to amino acid residues 15-25 on the longest isoform of human Tau.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2728542 (BioLegend Cat. No. 835203)
AB_2728543 (BioLegend Cat. No. 835204)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, casein kinase I isoform delta, serine/threonine-protein kinase Sgk1, Laforin, and alpha-synuclein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Castillo-Carranza DL, et al. 2014 J. Neurosci. 12:4260.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau, 15-25 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/28/2018

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