Purified anti-Sortilin Antibody

Pricing & Availability
Clone
W16078A (See other available formats)
Other Names
100 kD NT receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16078A_PURE_Sortilin_Antibody_1_032818
Western blot of purified anti-Sortilin antibody (clone W16078A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysates; Lane 4: 20 µg of mouse brain lysates. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). β-tubulin (Cat. No. 801206) was used as loading control. Enhanced chemiluminescence was used as the detection system.
  • W16078A_PURE_Sortilin_Antibody_1_032818
    Western blot of purified anti-Sortilin antibody (clone W16078A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysates; Lane 4: 20 µg of mouse brain lysates. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). β-tubulin (Cat. No. 801206) was used as loading control. Enhanced chemiluminescence was used as the detection system.
  • W16078A_PURE_Sortilin_Antibody_2_032818
    Western blot of purified anti-Sortilin antibody (clone W16078A). Lane 1: Molecular weight marker; Lane 2: 20 µg of SH-SY5Y cell lysate; Lane 3: 20 µg of Hela cell lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). β-tubulin (Cat. No. 801206) was used as loading control. Enhanced chemiluminescence was used as the detection system.
  • W16078A_PURE_Sortilin_Antibody_3_032818
    IHC staining of purified anti-Sortilin antibody (clone W16078A) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 2 µg/ml of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • W16078A_PURE_Sortilin_Antibody_4_032818
    IHC staining of purified anti-Sortilin antibody (clone W16078A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 2 µg/ml of the primary antibody 1 hour at room temperature. BioLegend´s Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
852101 25 µg $95.00
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852102 100 µg $235.00
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Description

Sortilin was originally purified from human brain tissue and was identified as a new lipoprotein receptor, which was later identified as a multifunctional sorting receptor of the VPS10P-domain receptor family. Although Sortilin is predominately expressed in neurons of the central and the peripheral nervous systems, it is also expressed in metabolic tissues including liver. Sortilin functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Sortilin is critical for lipid metabolism, and it has been implicated in lipid disorders. Sortilin is also an APOE receptor implicated in Alzheimer disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human SORT1 recombinant protein (327-638 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2728587 (BioLegend Cat. No. 852101)
AB_2728588 (BioLegend Cat. No. 852102)

Antigen Details

Structure
Sortilin is an 831 amino acid protein with a molecular mass of 95 kD.
Distribution

Tissue distribution: Sortilin is expressed in numerous tissues including brain, spinal cord, heart, skeletal muscle, thyroid, placenta, and testis.

Cellular distribution: Plamsa membrane, cytosol, endosome, endoplasmic reticulum, lysosome, and golgi apparatus.

Function
Sortilin functions as a cell surface receptor and mediates intracellular trafficking of diverse endogenous or exogenous protein substrates between the trans-Golgi network and plasma membrane compartments.
Interaction
Sortilin interacts with p75 Neurotrophin Receptor and is localized together with the golgi marker GM130 and the early endosome marker EEA1.
Ligand Receptor
Sortilin functions as lipoprotein receptor for neurotrophins and other growth factors.
Antigen References
  1. Skeldal S, et al. 2012. J. Biol. Chem. 287:43798-809.
  2. Herda S, et al. 2012. Immunity. 37:854-66.
  3. Gao A, et al. 2017. DNA Cell Biol. 36:1050-1061.
  4. Carlo AS. 2013. Prion. 7:378-82.
Gene ID
6272 View all products for this Gene ID
UniProt
View information about Sortilin on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 03/29/2018

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