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Purified anti-SMAD2 Antibody

Pricing & Availability
Clone
W21150C (See other available formats)
Regulatory Status
RUO
Other Names
MAD homolog 2, JV18-1, MAD-2, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a. W21150C_PURE_-SMAD2_WB_100923
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-SMAD2 (clone W21150C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight marker
  • a. W21150C_PURE_-SMAD2_WB_100923
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-SMAD2 (clone W21150C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight marker
  • b. W21150C_PURE_-SMAD2_ICFC_100923
    HeLa cells were fixed with fixation buffer (Cat. No. 420801), and permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401). Cells were then intracellularly stained with purified anti-SMAD2 (clone W21150C) (filled histogram) or rat IgG2b, κ isotype control (Cat. No. 400602) (open histogram, dashed line), followed by incubation with 0.1 µg/test of APC Goat anti-rat IgG (Cat. No. 405407) for 15 minutes at room temperature.
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624851 25 µg $165
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624852 100 µg $415
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Description

SMAD2 is a potent regulator of different cellular processes such as apoptosis, differentiation, and cell proliferation. SMAD2 is associated with SARA protein and the TGF-β receptor. SMAD2 gets phosphorylated by the TGF-β receptor in response to TGF-β signaling, which induces its dissociation from SARA and association with SMAD4 followed by translocation into the nucleus. In the nucleus, the SMAD2/SMAD4 protein complex participates in gene regulation by forming a transcription repressor complex in association with other cofactors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human SMAD2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.016 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone cross-reacts with mouse.

For intracellular flow cytometric staining with this clone, we recommended using Fixation Buffer (Cat. No. 420801), True-Phos™ Perm Buffer (Cat. No. 425401), Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) or Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not applicable.

We do not recommend this product for use in immunocytochemistry (ICC). 

Antigen Details

Structure
SMAD2 is a 467 amino acid protein with a predicted molecular weight of 52 kD
Distribution

Skeletal muscle, endothelial cells, heart and placenta; non-phosphorylated SMAD2 is present in the cellular cytoplasm close to the TGF-β receptor; phosphorylated SMAD2 is present in the cellular nucleus and binds to target promoter sequences.

Function
Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Interaction
TAZ/WWRT1, FOXH1, SNW1, CREB-binding protein (CBP), EP300, SNON, ALK4/ACVR1B, SKOR1, SKOR2, PRDM16, LEMD3, RBPMS, WWP1, PDPK1, DAB2, USP15, PPP5C, LDLRAD4, PMEPA1, ZFHX3, ZNF451, SMURF2, PPM1A, TGF-β, TGFBR1, TGIF, SMAD3, TRIM33, ZNF580, NEDD4, HGS, AIP1, WWP1, PML, ZNF8, RNF111, YAP1
Ligand/Receptor
SMAD2 is part of the TGF-β signaling pathway but it is not a ligand/receptor protein
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References
  1. Nakao A, et al. 1997. EMBO J. 16:5353–62.
  2. Attisano L. and Wrana JL, et al. 2002. Science. 296:1646-47.
  3. Moustakas A. et al. 2001. J Cell Sci. 114: 4359-69.
Gene ID
4087 View all products for this Gene ID
UniProt
View information about SMAD2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/09/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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