- 3F8 (See other available formats)
- Regulatory Status
- Other Names
- Serine/Threonine protein kinase PLK, Polo-like kinase (PLK), Serine-threonine protein kinase 13
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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PLK-1 (polo-like kinase 1) is a member of te serine/threonine protein kinase family, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila), PLK-1 contains two polo box domains with a predicted molecular weight of 68 kD. This nuclear protein is highly expressed in placenta and colon and has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK-1 may also be required for cell division; depletion of PLK-1 results in apoptosis. PLK-1 is upregulated by growth stimulating agents and is regulated by cell cycle position (highest in G2/M phase, declining to nearly undetectable levels after mitosis and throughout G1). PLK-1 is modified by phosphorylation (Thr210 is the major phosphorylation site in activated PLK-1 from mitotic cells) and has been shown to interact with nuclear distribution gene C. The 3F8 monoclonal antibody recognizes human and mouse PLK-1 and has been shown to be useful for Western blotting.Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Host Species
- Amino Acid: 300-603 of human PLK-1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
KO/KD-WB - Verified
IP, ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1.0 - 5.0 µg (1:100-1:500) antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_439756 (BioLegend Cat. No. 627701)
AB_439757 (BioLegend Cat. No. 627702)
- Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD
Nuclear protein, highly expressed in placenta and colon
- Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis
- Interacts with nuclear distribution gene C
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249.
2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793.
3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.
- Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1)
- Gene ID
- 5347 View all products for this Gene ID
- View information about PLK-1 on UniProt.org
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