Purified anti-PAX6 Antibody

Pricing & Availability
Clone
W19167A (See other available formats)
Other Names
Paired Box 6, Paired Box Protein PAX-6, Aniridia type II protein, Oculorhombin, AN2, D11S812E, MGDA, AN, MGC17209, WAGR, Aniridia 1, Aniridia 2, ASGD5, FVH1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19167A_PURE_PAX6_Antibody_1_092820.png
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-PAX6 antibody (clone W19167A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19167A_PURE_PAX6_Antibody_1_092820.png
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-PAX6 antibody (clone W19167A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19167A_PURE_PAX6_Antibody_2_092820.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-PAX6 antibody (clone W19167A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-PAX6 antibody (clone AD2.35) (Cat. No. 862002). Lane M: Molecular weight marker.
Cat # Size Price Quantity Avail. Save
939801 25 µg $100
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939802 100 µg $250
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Description

Paired box protein 6 (PAX6) is a transcription factor with dual roles as both transcriptional activator and repressor. It plays a critical role in the regulation of cell proliferation, cell cycle length and exit, and cell differentiation during neurogenesis and ocular, cortical, and olfactory development. PAX6 forms a complex with SOX2 and δ-crystallin enhancer DC5 to initiate lens placode development. In the central nervous system, PAX6 functions in the regulation of progenitor cell proliferation, telencephalic patterning, cortical layer formation, and the maintenance of balance in neuron differentiation.   Heterozygous mutations in PAX6 can result in aniridia, nystagmus, neurodevelopmental disorders, and auditory and memory impairment. In the neuroendocrine system, PAX6 functions in the development of β cells, pancreatic islet of Langerhans cells, and the pituitary gland as well as in insulin biosynthesis, and glucose-induced insulin secretion. PAX6 functions as a tumor suppressor in prostate cancer and glioblastoma by inhibiting androgen receptors.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide corresponding to C-terminus of human PAX6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 0.25 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

At higher concentrations, this clone recognizes a ~100 kD protein of unknown origin. This protein was not detected in W19167A immunoprecipitates that were probed by western blot using W19167A.

RRID
AB_2888904 (BioLegend Cat. No. 939801)
AB_2888904 (BioLegend Cat. No. 939802)

Antigen Details

Structure
PAX6 is a 422 amino acid protein with a predicted molecular weight of 47 kD.
Distribution

Expressed in the eye, nose, brain pancreas/Nucleoplasm

Function
Transcriptional regulation, differentiation
Antigen References

1. Gosmain Y, B, et al. 2012. Mol.. Endocrinol. 26:696
2. Kamachi Y, et al. 2001. Genes Dev. 10:1272
3. Manuel M, et al. 2015. Fron. Cell. Neurosci. 9:70
4. Shyr C, et al. 2010. Prostate. 274:6056

Gene ID
5080 View all products for this Gene ID
UniProt
View information about PAX6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/28/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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